Dr Toney Pacey-Miller BSc(MACQU), BAppSc(Hon)(SCU), GradDipEd(SCU), PhD(SCU) Toni presently holds the position of Associate Research Fellow involved with the Grain Foods CRC on Milling wheat and was previously involved in the CRC's "Malting Barley" project. These project involve a genomics approach to wheat and barley improvement. This will ultimately identify superior genotypes for immediate commercial use. Toni has worked previously within the Centre as a Senior Scientific Officer involved in the "Development of High Throughput Genotyping" project. This project developed new efficient ways for the discovery and analysis of large numbers of single nucleotide polymorphisms (SNPs). High throughput methods for screening SNPs have potential application in a wide range of research and diagnostic projects. The technologies developed in this project will be crucial in effective application of the benefits of genome research in the analysis of biodiversity and in the application of genotyping for the genetic improvement of agricultural species. Toni has developed a single stranded method for pyrosequencing which utilises the use of a "tag" system. This increases efficiency and decreases costs involved with the pyrosequencing process. Prior to commencing on her this role, Toni completed her PhD at the Centre on "Changes in Gene Expression Associated with the end of Dormancy in Grapes". The project determined patterns of gene expression as the grapevine approaches the end of dormancy and through into bud burst. Although a single genetic trigger was not found the data collected in this study increased the knowledge of dormant grape gene expression immensely. Microarray technology was used for this purpose and a bud EST library was produced.
Journal articles
Differential LongSAGE tag abundance analysis in a barley seed germination time course and validation with relative real-time RT-PCR (with Jessica F. White, Peter C. Bundock, and Robert J. Henry), Plant Science (2008)
A Long Serial Analysis of Gene Expression (LongSAGE) approach was employed to identify changes in...
Abundant transcripts of malting barley identified by serial analysis of gene expression (SAGE) (with Jessica F. White, Allison C. Crawford, Giovanni M. Cordeiro, Daniel Barbary, Peter C. Bundock, and Robert J. Henry), Plant Biotechnology Journal (2006)
Serial analysis of gene expression (SAGE) was applied to the major cereal crop barley (Hordeum...
DNA Banks and their role in facilitating the application of genomics to plant germplasm (with Nicole F. Rice, Giovanni M. Cordeiro, Mervyn Shepherd, Peter C. Bundock, Louis MT Bradbury, Loraine Watson, Allison C. Crawford, Agnelo Furtado, and Robert J. Henry), Plant Genetic Resources (2006)
Advances in genomics have provided technologies for high throughput analysis of plant genomes with potential...
A universal protocol for identification of cereals (with Shane R. McIntosh and Robert J. Henry), Journal of Cereal Science (2005)
Plant fingerprinting and identification has increasingly become a focus in commerce and manufacturing with an...
Consistent production of cost-effective LongSAGE libraries (with Allison C. Crawford, Jessica F. White, Peter C. Bundock, Giovanni Cordeiro, Shane R. McIntosh, Lee Rooke, and Robert J. Henry), Plant Molecular Biology Reporter (2005)
Serial analysis of gene expression (SAGE) and related techniques are gaining popularity as tools for...
Conference publications
Custom wheat microarray development for analysis of grain quality (with Peter C. Bundock and Robert J. Henry), 11th International Wheat Genetics Symposium (2008)
Many genes influencing wheat grain quality are expressed during seed development. Custom microarrays have been...
Grain quality analysis using a custom wheat microarray (with Peter C. Bundock and Robert J. Henry), 58th Australian Cereal Chemistry Conference (2008)
Custom microarrays can be used to examine genes influencing wheat grain quality that are expressed...
Characterisation of the malting barley transcriptome (with Jessica F. White, Allison C. Crawford, Peter C. Bundock, Giovanni M. Cordeiro, and Robert J. Henry), 13th Australian Barley Technical Symposium (2007)
The introduction of large-scale gene expression technologies such as serial analysis of gene expression (SAGE)...
Rapid malt - the path to faster malting barley (with Jessica F. White, Peter C. Bundock, and Robert J. Henry), 13th Australian Barley Technical Symposium (2007)
Malt analysis provides guidance on the effectiveness of the malting process and the suitability of...
Understanding gene expression in the developing grain (with Loraine Watson, Jessica F. White, Shane R. McIntosh, Allison C. Crawford, Peter C. Bundock, Daniel Barbary, Giovanni M. Cordeiro, and Robert J. Henry), 57th Australian Cereal Chemistry Conference (2007)
Serial Analysis of Gene Expression (SAGE) has been used to understand more comprehensively the gene...