Our laboratory is interested in the processes of DNA replication, Recombination and Cell Division in E. coli. In particular, we are interested in how these processes are coordinated. It has been recently shown that replication forks stop for a variety of mundane, housekeeping reasons as they replicate chromosomes. This happens both in prokaryotic and eukaryotic cells. After stopping, these forks need to get repaired. This is thought to occur predominately by homologous recombination. Then after repair, these forks need to get up and running again. A special set of proteins call the Replication Restart Proteins form a multi-subunit complex at a repaired replication fork that helps to reload the replisome. In E. coli, the Replication Restart Proteins include PriA, PriB, PriC, DnaT, DnaC, and DnaB.
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Differential Requirements of Two recA Mutants for Constitutive SOS Expression in Escherichia coli K-12 (with Jarukit Edward Long, Nicholas Renzette, and Richard Centore), PLoS One (2008)
Background Repairing DNA damage begins with its detection and is often followed by elicitation of...
Allele specific synthetic lethality between priC and dnaAts alleles at the permissive temperature of 30°C in E. coli K-12 (with Tania Hinds), BMC Microbiology (2004)
Background DnaA is an essential protein in the regulation and initiation of DNA replication in...
Biochemical and Genetic Characterization of PpcA, a Periplasmic c-Type Cytochrome in Geobacter Sulfurreducens (with Jon R. Lloyd, Allison L Hodges Myerson, Ching Leang, Maddalena V. Coppi, Stacey Cuifo, Barbara A. Methѐ, and Derek Lovley), Biochemical Journal (2003)
A 9.6 kDa periplasmic c -type cytochrome, designated PpcA, was purified from the Fe(III)-reducing bacterium...
Development of a Genetic System for Geobacter Sulfurreducens (with Maddalena V. Coppi, Ching Leang, and Derek Lovley), Applied and Environmental Microbiology (2001)
Members of the genus Geobacter are the dominant metal-reducing microorganisms in a variety of anaerobic...
recA-Like Genes From Three Archaean Species with Putative Protein Products Similar to Rad51 and Dmcl Proteins of the Yeast Saccharomyces Cerevisiae (with Leslie H. Satin, Hardeep Sarma, and Alvin J. Clark), Nucleic Acids Research (1996)
The process of homologous recombination has been documented in bacterial and eucaryotic organisms. The Escherichia...