Mango is highly sensitive to soil salinity and alkalinity therefore, its expansion to larger areas suitable to for cultivation is restricted. The Central Institute for Subtropical Horticulture (CISH), Lucknow, in its Vision 2030 under strategy and framework is making efforts for the development and standardisation of rootstocks for abiotic stress tolerance. For this, the new emerging field in 'omics' called 'phenomics' could suitably be employed for the measurement of phenomes (physical and biochemical traits) which are function of genetic makeup and environmental influence. Phenotyping of mango rootstock and scion traits for stress tolerance would help in selection of suitable rootstock and a suitably compatible scion. Among the various phenomes, membrane stability is widely used parameter to screen out the germplasm for stress tolerance. The plants when exposed to abiotic stress conditions, reactive oxygen species levels would be increased rapidly in plant cells causing the loss of membrane integrity. This would make loss of selective permeability and eventually lead to apoptosis and cell death. Therefore, the genotypes which have capability to maintain high membrane integrity would also have increased tolerance to oxidative stress caused by various stress conditions. At CISH, the rootstock seedlings which were collected from various locations in India are evaluated for the membrane integrity using membrane stability index (MSI) protocol. The results showed that the membrane stability varied among the genotypes and it was in the range of 58% - 86% when studied at the open control conditions. From the values, the genotypes could be grouped into three different categories with low, moderate and high stability groups viz., Ratnagir, Nattumanga, Kulambe as low (<70%); IC70676, Puliyan, Moovandan, Vellaicolumban, Kurukkan, Bappakai, Mylepalion, Trissur as moderate (>70% but <80%) and Goa, Komanga, 202224, Nakkare, Olour, 676 as high (>80%). Among the genotypes, 676 were having higher MSI (86.36%) whereas Ratnagir low (58.62%). Though several factors affects membrane integrity, this parameter would definitely provide some insight to screen out the mango rootstocks and scion. Added to these intrinsic stress tolerance traits, various other traits like high wax content of leaves, high osmolarity, high ion homeostasis and high chlorophyll florescence would be very helpful in phenotyping mango for stress tolerance traits.