We present an experimental investigation of fracture in self-assembled gold nanoparticle mono- and multilayers attached to elastomer substrates and subjected to tensile stress. Imaging the fracture patterns down to the scale of single particles provides detailed information about the crack width distribution and allows us to compare the scaling of the average crack spacing as a function of strain with predictions by shear-lag models. With increasing particle size, the fracture strength is found to increase while it decreases as the film thickness is built up layer by layer, indicating stress inhomogeneity in the thickness dimension.