A high-resolution image reconstruction algorithm previously used to improve undersampled infrared airborne imagery was applied to two different sets of digital microscopy images. One set is that of medical pap smear images, and the second set contains metallurgical micrographs. Both the pap smear images and the metallurgical micrographs are undersampled, thus causing loss of detail and aliasing artifacts. The algorithm minimizes the effects of aliasing and restores detail unobtainable through simple interpolation techniques. Both applications demonstrate improvement by use of the image reconstruction algorithm.