This annual report (2015-2016) provides a continuing view on the position of the Cushing/Whitney Medical Library End-user Bioinformatics Program. Besides the report on the three main areas of training, resources and tools, and consultations, it contains the results of the recent assessment “Information and Needs Assessment for Biomedical Research in the Omics Era” During this period, 741 Yale affiliates attended (out of 1240 registered) the end-user bioinformatics training and presentations organized by the Medical Library.  This year, the number of Ingenuity Pathway Analysis and MetaCore accounts continued to grow. Consequently, the number and length of research support consultations (130 researchers benefited) on these tools also increased. In addition, the Medical Library added the professional version of the Human Gene Mutation Database, and Biocyc database, and it has begun license Partek Flow as part of a pilot project to support the secondary stage of the omics high throughput data analysis cycle. According to the aforementioned assessment, the data and information needs differ between graduate students, postdocs, and faculty. These and other results are discussed in this report.