Effects of primers and Taq polymerase on randomly amplified polymorphic DNA analysis for typing Listeria monocytogenes from the environment of a shrimp processing plant
Ninety-nine randomly selected isolates of Listeria monocytogenes obtained during a 5 month sampling period from several processing environment locations in a shrimp processing plant were subjected to RAPD analysis with the use of 4 primers. Preliminary studies indicated that the number of DNA bands and their intensity differed greatly with respect to the commercial source of the Taq polymerase used with individual isolates. 18 composite RAPD types were discerned with the use of the 4 primers. Among these 18 composite RAPD types, type 1 comprised 14 indistinguishable isolates and type 9 comprised 49 indistinguishable isolates. Results indicated that the shrimp processing plant was dominated by these 2 RAPD types, which comprised 63.6% of the 99 randomly selected isolates.
Robert E. Levin, J. Cao, C. Cromnin, and L. McLandsborough. "Effects of primers and Taq polymerase on randomly amplified polymorphic DNA analysis for typing Listeria monocytogenes from the environment of a shrimp processing plant" Food Biotechnology 19.3 (2005): 217-226.
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