Copyright (c) 2009 All rights reserved. http://works.bepress.com/robert_henry Recent documents in Robert J Henry en-us Wed, 04 Nov 2009 23:18:47 PST 3600 http://works.bepress.com/robert_henry/782 http://works.bepress.com/robert_henry/782 Tue, 03 Nov 2009 19:21:50 PST A McLauchlan http://works.bepress.com/robert_henry/781 http://works.bepress.com/robert_henry/781 Tue, 03 Nov 2009 19:21:47 PST L Slade Lee http://works.bepress.com/robert_henry/780 http://works.bepress.com/robert_henry/780 Tue, 03 Nov 2009 19:21:45 PST Mervyn Shepherd http://works.bepress.com/robert_henry/779 http://works.bepress.com/robert_henry/779 Tue, 03 Nov 2009 19:21:43 PST Robert J. Henry http://works.bepress.com/robert_henry/778 http://works.bepress.com/robert_henry/778 Tue, 03 Nov 2009 19:21:40 PST A McLauchlan http://works.bepress.com/robert_henry/777 http://works.bepress.com/robert_henry/777 Tue, 03 Nov 2009 19:21:38 PST Mervyn Shepherd http://works.bepress.com/robert_henry/776 http://works.bepress.com/robert_henry/776 Tue, 03 Nov 2009 19:21:36 PST Robert J. Henry http://works.bepress.com/robert_henry/775 http://works.bepress.com/robert_henry/775 Tue, 03 Nov 2009 19:21:33 PST Wendy R. Lawson http://works.bepress.com/robert_henry/774 http://works.bepress.com/robert_henry/774 Tue, 03 Nov 2009 19:21:31 PST Robert J. Henry http://works.bepress.com/robert_henry/773 http://works.bepress.com/robert_henry/773 Tue, 03 Nov 2009 19:21:29 PST Maurizio Rossetto http://works.bepress.com/robert_henry/772 http://works.bepress.com/robert_henry/772 Tue, 03 Nov 2009 19:21:26 PST Genotype identification in cereals is important as different varieties are suitable for different end-uses, e.g. malt vs. feed in barley, bread vs. pasta or noodles in wheat, aromatic vs. non-aromatic rice. Several PCR methods were compared for their suitability in providing DNA fingerprints for distinguishing cereal varieties from one another or to study the genetic diversity within a species. These included the use of RAPD-PCR using arbitrary primers and PCR using primers based on barley a-amylase genes and microsatellites. Reliable identification of cereal species is potentially useful for determination of the composition of admixtures or processed products. A simple method was developed for determining the presence or absence of the most important cereal species. This was achieved by designing species specific 'nested' primers from the intergenic 5S ribosomal RNA spacer region, consisting of 20-24 bp oligonucleotides. PCR using these primers in combination with one of the consensus 5S rRNA primers gave consistent banding profiles within a species using DNA samples extracted from a range of materials. Its application in processed foods is yet to be determined. Several methods for the preparation of DNA samples from leaves, embryos and flour (ground seeds) suitable for use in PCR were compared, with the aim of determining the simplest and fastest reliable method for use in routine sample analysis. Hian-Lien Ko http://works.bepress.com/robert_henry/771 http://works.bepress.com/robert_henry/771 Tue, 03 Nov 2009 19:21:24 PST L Slade Lee http://works.bepress.com/robert_henry/770 http://works.bepress.com/robert_henry/770 Tue, 03 Nov 2009 19:21:21 PST Mahin Abedinia http://works.bepress.com/robert_henry/769 http://works.bepress.com/robert_henry/769 Tue, 03 Nov 2009 19:21:19 PST Robert J. Henry http://works.bepress.com/robert_henry/768 http://works.bepress.com/robert_henry/768 Tue, 03 Nov 2009 19:21:17 PST David ME Poulsen http://works.bepress.com/robert_henry/767 http://works.bepress.com/robert_henry/767 Tue, 03 Nov 2009 19:21:14 PST Mervyn Shepherd http://works.bepress.com/robert_henry/766 http://works.bepress.com/robert_henry/766 Tue, 03 Nov 2009 19:21:12 PST Robert J. Henry http://works.bepress.com/robert_henry/765 http://works.bepress.com/robert_henry/765 Tue, 03 Nov 2009 19:21:09 PST Tina L. Maguire http://works.bepress.com/robert_henry/764 http://works.bepress.com/robert_henry/764 Tue, 03 Nov 2009 19:21:07 PST M P. Jobin-Decor http://works.bepress.com/robert_henry/763 http://works.bepress.com/robert_henry/763 Tue, 03 Nov 2009 19:21:05 PST J E. Vickers http://works.bepress.com/robert_henry/762 http://works.bepress.com/robert_henry/762 Tue, 03 Nov 2009 19:21:03 PST Hian-Lien Ko http://works.bepress.com/robert_henry/761 http://works.bepress.com/robert_henry/761 Tue, 03 Nov 2009 19:21:00 PST David ME Poulsen http://works.bepress.com/robert_henry/760 http://works.bepress.com/robert_henry/760 Tue, 03 Nov 2009 19:20:58 PST R J. Marschke http://works.bepress.com/robert_henry/759 http://works.bepress.com/robert_henry/759 Tue, 03 Nov 2009 19:20:55 PST Many routine analyses of plants require the preparation or extraction of nucleic acid for PCR analysis. We have systematically evaluated the options for a single step protocol for the preparation of plant material. The objective was to establish the optimum composition of a solution in which a small sample of the plant tissue could be heated to prepare an extract that was suitable for direct introduction into a PCR. Heating tissue in 100 mM Tris-HCl, pH 7.4, 1 M KCl, 10 mM EDTA for 10 minutes at 95oC was found to give good results in a wide range of applications. The method was used for PCR analysis of single and multicopy genes, for RAPD analysis, for confirmation of transgenes and for analysis of DNA and RNA viruses in plant tissue. Samples successfully analysed included fresh, frozen and dried plant material, a wide range of plant species and plant tissues. Darelle Thomson http://works.bepress.com/robert_henry/758 http://works.bepress.com/robert_henry/758 Tue, 03 Nov 2009 19:20:53 PST S Weining http://works.bepress.com/robert_henry/757 http://works.bepress.com/robert_henry/757 Tue, 03 Nov 2009 19:20:51 PST M Mathews http://works.bepress.com/robert_henry/756 http://works.bepress.com/robert_henry/756 Tue, 03 Nov 2009 19:20:49 PST S Weining http://works.bepress.com/robert_henry/755 http://works.bepress.com/robert_henry/755 Tue, 03 Nov 2009 19:20:47 PST Carl Rathus http://works.bepress.com/robert_henry/754 http://works.bepress.com/robert_henry/754 Tue, 03 Nov 2009 19:20:44 PST Wendy R. Lawson