Evaluation of plasma time-activity curves of technetium-99M-mebrofenin for measurement of hepatic function in dogs.
In 9 healthy dogs in which hepatic damage was induced using using a hepatotoxic agent, plasma samples were collected 1, 3, 5, 7, 9, 15, 20, 30, 40, 50, and 60 min following a peripheral venous injection of 111-222 MBq (3-6 mCi) of 99mTc-mebrofenin. The area under the plasma time-activity curve (AUC) was calculated using 2 methods and compared to direct measurement of the hepatic extraction efficiency. First pass hepatic extraction efficiency of 99mTc-mebrofenin was calculated from differential equation analysis of a 2-compartment model following mesenteric venous injection of the radiopharmaceutical. In 7 of the original 9 dogs and 2 additional healthy dogs, plasma clearance and hepatic extraction efficiency measurements were repeated following induction of hepatic injury by thiacetarsamide (3 mg/kg i.v. twice daily for 1 day). In one additional dog, hepatic injury was induced using carbon tetrachloride (0.3 ml/kg i.p.). Plasma time-activity curves of 99mTc-mebrofenin had kinetics of a 2 compartment model. Area under the curve was highly correlated with hepatic extraction efficiency. The AUC integrated from 1 to 60 min (AUC60) had the best correlation with hepatic extraction efficiency (r=0.978). A formula for calculation of hepatic extraction efficiency was derived using linear regression analysis. It is concluded that plasma clearance of a peripheral venous injection of 99mTc-mebrofenin is a simple, non-invasive, convenient method to measure hepatic function without a gamma camera. Accession Number: 20002210034
C. L. Matwichuk, G. B. Daniel, Robert C. DeNovo Jr., A. E. Shultze, D. E. Schmidt, and K. E. Creevy. "Evaluation of plasma time-activity curves of technetium-99M-mebrofenin for measurement of hepatic function in dogs." Veterinary Radiology & Ultrasound 41.1 (2000): 78-84.
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