Handout
qPCR analysis of differential gene expression (wet lab)
Advanced Molecular Biology
(2016)
Description
This class tested protocol will guide students through the steps for the following activities:
- Analyze qPCR data to determine normalized gene expression for candidate genes identified from an RNA-Seq data set
- Set up additional or repeat experimental qPCR reactions
- Create a draft poster outlining the methods & data collected during the 6-week RNA-Seq analysis lab module
Keywords
- qPCR,
- RNA-seq,
- active learning,
- gene expression analysis protocol
Disciplines
Publication Date
June, 2016
Citation Information
Raymond A Enke. "qPCR analysis of differential gene expression (wet lab)" Advanced Molecular Biology (2016) Available at: http://works.bepress.com/raymond_enke/75/
Creative Commons license
This work is licensed under a Creative Commons CC_BY-SA International License.