Handout
qPCR Primer Standard Curve Assay (wet lab) + KEGG Pathway analysis (computational)
Bio 480 Advanced Molecular Biology
(2016)
Description
This class tested protocol will guide students through the steps for the following activities:
- analyzing qPCR standard curve data to determine primer efficiency
- analyzing differential gene expression experimental qPCR data
- applying KEGG pathway analysis of selected candidates genes
Keywords
- qPCR,
- RNA-Seq,
- active learning,
- molecular biology protocol,
- KEGG pathways
Disciplines
Publication Date
Fall October 31, 2016
Citation Information
This work is licensed under a Creative Commons Attribution-ShareAlike 3.0 United States License. Recommended citation: Enke, R. (2016) Setting up a qPCR Primer Standard Curve Assay. CSHL DNALC RNA-Seq for the Next Generation Working Group. http://www.rnaseqforthenextgeneration.org/profiles/raymond-enke.html#teaching
Creative Commons license
This work is licensed under a Creative Commons CC_BY-SA International License.