Article
High-field 19.6 T 27Al Solid-state MAS NMR of in vitro Aluminated Brain Tissue
Journal of Magnetic Resonance
(2003)
Abstract
The combination of 27Al high-field solid-state NMR (19.6 T) with rapid spinning speeds (17.8 kHz) is used to acquire 27Al NMR spectra of total RNA human brain temporal lobe tissues exposed to 0.10 mM Al3+ (as AlCl3) and of human retinal pigment epithelial cells (ARPE-19), grown in 0.10 mM AlCl3. The spectra of these model systems show multiple Al3+ binding sites, good signal/noise ratios and apparent chemical shift dispersions. A single broad peak (−3 to 11 ppm) is seen for the aluminated ARPE-19 cells, consistent with reported solution-state NMR chemical shifts of Al-transferrin. The aluminated brain tissue has a considerably different 27Al MAS NMR spectrum. In addition to the transferrin-type resonance, additional peaks are seen. Tentative assignments include: −9 to −3 ppm, octahedral AlO6 (phosphate and water); 9 ppm, condensed AlO6 units (Al–O–Al bridges); 24 ppm, tetrahedral AlO3N and/or octahedral Al–carbonate; and 35 ppm, more N-substituted aluminum and /or tetrahedral AlO4. Thus, brain tissue is susceptible to a broad range of coordination by aluminum. Furthermore, the moderate 27Al CQ values (all less than 10 MHz) suggest future NMR studies may be performed at 9.4 T and a spin rate of 20 kHz.
Keywords
- Al solid-state NMR,
- Human brain,
- RNA,
- Aluminum coordination,
- Alzheimer's disease
Disciplines
Publication Date
October, 2003
Citation Information
Pamela L. Bryant, Walter J. Lukiw, Zhehong Gan, Randall W. Hall, et al.. "High-field 19.6 T 27Al Solid-state MAS NMR of in vitro Aluminated Brain Tissue" Journal of Magnetic Resonance Vol. 170 Iss. 2 (2003) p. 257 - 262 ISSN: 1090-7807 Available at: http://works.bepress.com/randall_hall/9/