Determination of propofol using high performance liquid chromatography in whole blood with fluorescence detection.
High-performance liquid chromatography method for the determination of propofol has been developed and validated. Following a liquid extraction using ethyl acetate and hexane, samples were separated by reverse-phase high-performance liquid chromatography on an XBridge C(18) column and quantified using fluorescence detection at an excitation of 276 nm and an emission of 310 nm. The mobile phase was a mixture of water (pH 4.0) and acetonitrile, with a flow rate of 1.5 mL/min. The standard curve ranged from 5-2000 ng/mL. Intra- and inter-assay variability for propofol was less than 10%, and the average recovery was greater than 95%. This assay is suitable for use in pharmacokinetic studies.
J. Yarbrough, Ralph C. Harvey, and Sherry Cox. "Determination of propofol using high performance liquid chromatography in whole blood with fluorescence detection." Journal of Chromotographic Science 50.3 (2012): 162-166.
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