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Selected Works of Rajesh Nagarajan
Presentation
Optimization of Multiplex PCR for Multiple Locus Variable Number Tandem Repeat Analysis for Salmonella typhimurium
College of Arts and Sciences Poster Presentations
  • George Hafez, Department of Chemistry and Biochemistry, Boise State University
  • Remington Turner, Department of Chemistry and Biochemistry, Boise State University
  • Andrea Haskett, Department of Chemistry and Biochemistry, Boise State University
  • Rajesh Nagarajan, Department of Chemistry and Biochemistry, Boise State University
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Document Type
Student Presentation
Publication Date
4-11-2011
Disciplines
Abstract

Salmonella typhimurium infects an estimated 1.4 million in the United States and kills over five hundred annually. The current way of categorization, Pulse Field Gel Electrophoresis (PFGE) and antibiotic sensitivity testing, lack the ability to distinguish between some closely related strains. We are attempting to optimize a new technique of categorization, Multiple Locus Variable number tandem repeat Analysis (MLVA). We made changes to temperature and concentration variables within the multiplex PCR procedure to streamline the identification process. By optimizing the multiplex PCR procedure we intend to create more consistent amplification of the loci used in MLVA analysis, which will improve efficiency in differentiating S. typhimurium isolates.

Faculty Sponsor
Dr. Rajesh Nagarajan
Citation Information
George Hafez, Remington Turner, Andrea Haskett and Rajesh Nagarajan. "Optimization of Multiplex PCR for Multiple Locus Variable Number Tandem Repeat Analysis for Salmonella typhimurium" (2011)
Available at: http://works.bepress.com/rajesh_nagarajan/15/