Article
The new genetic environment of cfr on plasmid pBS-02 in a Bacillus strain
Journal of Antimircobial Chemotherapy
Document Type
Article
Disciplines
Publication Version
Published Version
Publication Date
5-1-2011
DOI
10.1093/jac/dkr037
Abstract
Sir,
The gene cfr, encoding a 23S rRNA methyltransferase, confers resistance to five chemically unrelated antimicrobial classes, including phenicols, lincosamides, oxazolidinones, pleuromutilins and streptogramin A (PhLOPSA), and has been observed mainly in staphylococcal isolates over the past decade.1–3 Our previous study reported a cfr-carrying plasmid, pBS-01, in a novel strain (BS-01) of a Bacillus species isolated from a pig farm in China.4 In our routine surveillance study on antimicrobial resistance in farm animals in 2010, another florfenicol and chloramphenicol-resistant Bacillus species strain (named BS-02) from swine faeces was identified. Gram staining, sequence analysis of the 16S rRNA and API 50CH testing associated with the API 20E system (bioMérieux, France) showed that BS-02 had an identical profile to that of strain BS-01, which suggested that BS-02 also belongs to a novel Bacillus species. Despite their identical profiles, more than six PFGE band differences were observed between BS-01 and BS-02 (data not shown), indicating that they belong to different clonal types of the same species.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Wan-Jiang Zhang, Cong-Ming Wu, Yang Wang, Zhang-Qi Shen, et al.. "The new genetic environment of cfr on plasmid pBS-02 in a Bacillus strain" Journal of Antimircobial Chemotherapy Vol. 66 Iss. 5 (2011) p. 1174 - 1175 Available at: http://works.bepress.com/qijing-zhang/27/
This article is published as Zhang, Wan-Jiang, Cong-Ming Wu, Yang Wang, Zhang-Qi Shen, Lei Dai, Jing Han, Steven L. Foley, Jian-Zhong Shen, and Qijing Zhang. "The new genetic environment of cfr on plasmid pBS-02 in a Bacillus strain." Journal of antimicrobial chemotherapy 66, no. 5 (2011): 1174-1175. doi: 10.1093/jac/dkr037. Posted with permission.