"Hydrophobic Probe Binding of β-Lactoglobulin in the Native and Molten Globule State Induced by High Pressure as Affected by pH, KIO3 and N-Ethylmaleimide" by Jian Yang

Article

Hydrophobic Probe Binding of β-Lactoglobulin in the Native and Molten Globule State Induced by High Pressure as Affected by pH, KIO3 and N-Ethylmaleimide

Journal of Agricultural and Food Chemistry (2002)

Jian Yang, Washington State University
Joseph R. Powers, Washington State University
Stephanie Clark, Washington State University
A. Keith Dunker, Washington State University
Barry G. Swanson, Washington State University

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Abstract

High hydrostatic pressure (HHP) at 500 MPa and 50 °C induces β-LG into the molten globule state. Retinol, cis-parinaric acid (CPA), and 1-anilino-naphthalene-8-sulfonate (ANS) fluorescence from pH 2.5 to 10.5 in the presence of the native and molten globule states of β-LG indicate that retinol binds to β-LG in the calyx, CPA at the surface hydrophobic site, and ANS in multiple hydrophobic sites. HHP treatment results in a decrease of β-LG affinity for retinol and CPA, suggesting conformational changes in the calyx and surface hydrophobic site of β-LG during HHP treatment. β-LG treated by HHP in the presence of N-ethylmaleimide (NEM) retains retinol affinity, suggesting that NEM protects the calyx conformation of β-LG during HHP treatment. HHP treatment of β-LG in the presence of KIO3 exhibits a great decrease of CPA affinity compared to HHP-treated β-LG in the absence of KIO3, suggesting the formation of non-native disulfide bonding at the CPA binding site.

Keywords

β-Lactoglobulin,
high pressure,
molten globule,
retinol,
cis-parinaric acid,
1-anilino-naphthalene-8-sulfonate,
N-ethylmaleimide,
KIO3

Disciplines

Publication Date

August 3, 2002

Publisher Statement

Reprinted with permission from Journal of Agricultural and Food Chemistry, J. Yang, J.R. Powers, S. Clark, A.K. Dunker, B.G. Swanson, 50(18): 5207-5214. doi10.1021/jf020221j . Copyright 2002 American Chemical Society."

Citation Information

Jian Yang, Joseph R. Powers, Stephanie Clark, A. Keith Dunker, et al.. "Hydrophobic Probe Binding of β-Lactoglobulin in the Native and Molten Globule State Induced by High Pressure as Affected by pH, KIO3 and N-Ethylmaleimide" Journal of Agricultural and Food Chemistry Vol. 50 Iss. 18 (2002)
Available at: http://works.bepress.com/milkmade_stephanie_clark/2/