Publications

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Serologic survey of felids in the Peten region of Guatemala (with Adrienne Lickey, Sharon Patton, and Edward C. Ramsay), Journal of Zoo and Wildlife Medicine (2005)

Blood samples were analyzed from 30 domestic cats (Felis domesticus) from the Petén region of...

 

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PCR prevalence of Ranavirus in free-ranging eastern box turtles (Terrapene carolina carolina) at rehabilitation centers in three southeastern US states (with Matthew C. Allender, M Abd-Eldaim, Juergen P. Schumacher, D McRuer, and L S. Christian), Journal of wildlife diseases (2011)

Ranaviruses (genus Ranavirus) have been observed in disease epidemics and mass mortality events in free-ranging...

 

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Sensitivity and specificity of a nested polymerase chain reaction for detection of lentivirus infection in lions (Panthera leo) (with H Adams, M van Vuuren, Stephen Kania, A M. Bosman, D Keet, and J New), Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians (2010)

Feline immunodeficiency virus (FIV) is a lentivirus in the Retroviridae family that causes lifelong infection...

 

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Bovine Viral Diarrhea Infections in New World Camelids—A Review (with Sarel R. Van Amstel), Small Ruminant Research (2010)

Bovine virus diarrhea virus (BVDV) has recently been identified as an important infectious disease of...

 

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An update on Feline Infectious Peritonitis, Veterinary Medicine (2009)

CONCLUSION FIP is an enigmatic disease that can frustrate clinicians and distress cat owners. It...

 

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Influenza virus A (H1N1) in giant anteaters (Myrmecophaga tridactyla (with S Nofs, M Abd-Eldaim, K V. Thomas, David Toplon, and D Rouse), Emerging infectious diseases (2009)

In February 2007, an outbreak of respiratory disease occurred in a group of giant anteaters...

 

Absence of Frog Virus 3 and Herpesvirus in a PCR Survey of Two Aquatic Turtle Species from a Threatened Habitat in Illinois. (with Matthew C. Allender, Mohammed Abd-Eldaim, and Andrew Kuhns), Journal of Herpetological Medicine and Surgery (2009)
 

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Detection of feline panleukopenia virus using a commercial ELISA for canine parvovirus (with M Abd-Eldaim and M Beall), Veterinary therapeutics : research in applied veterinary medicine (2009)

Feline panleukopenia virus (FPV) is a significant pathogen of cats. Rapid virus detection is critical...

 

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Development and validation of a TaqMan real-time reverse transcription-PCR for rapid detection of feline calicivirus (with M M. Abd-Eldaim, Rebecca P. Wilkes, and K V. Thomas), Archives of virology (2009)

Feline calicivirus (FCV) is a common cause of upper respiratory tract disease in cats and...

 

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The epidemiology of lion lentivirus among a population of free-ranging lions (Panthera leo) in Kruger National Park, Republic of South Africa (with H Adams, M van Vuuren, A Bosman, D Keet, and John C. New Jr.), Journal of South African Veterinary Association (2009)

Feline immunodeficiency virus is a lentivirus of domestic cats that causes significant lifelong infection. Infection...

 

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Global food systems and public health : production methods and animal husbandry, A National Commission on Industrial Farm Animal Production Report. Pew Commission on Industrial Farm Animal Production (with M Salman, John C. New Jr., M Bailey, Corrie Brown, L Detwiler, D Galligan, C Hall, G Lonergan, L Mann, D Renter, M Saeed, B White, and S Zika), John C. New Jr., DVM, MPH, DACVPM (2008)
 

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Evaluation of antibodies against feline coronavirus 7b protein for diagnosis of feline infectious peritonitis in cats (with M Abd-Eldaim, S E. Zika, Joseph M. Mankin, and Stephen A. Kania), American Journal of Veterinary Research (2008)

OBJECTIVE: To determine whether expression of feline coronavirus (FCoV) 7b protein, as indicated by the...

 

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Molecular phylogeny of equine herpesvirus 1 isolates from onager, zebra and Thomson's gazelle (with Y M. Ghanem, H Fukushi, E S. Ibrahim, K Ohya, and T Yamaguchi), Archives of virology (2008)

Viruses related to equine herpesvirus type 1 (EHV-1) were isolated from an aborted fetus of...

 

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Genetic relatedness and pathogenicity of equine herpesvirus 1 isolated from onager, zebra and gazelle (with E S. Ibrahim, M Kinoh, T Matsumura, G P. Allen, T Yamaguchi, and H Fukushi), Archives of virology (2007)

Equine herpesvirus 1 was isolated from an onager in 1985, a zebra in 1986 and...

 

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Analysis of genetic mutations in the 7a7b open reading frame of coronavirus of cheetahs (Acinonyx jubatus) (with E Moore, Rebecca P. Wilkes, S B. Citino, and Stephen A. Kania), American Journal of Veterinary Research (2006)

OBJECTIVE: To analyze the 7a7b genes of the feline coronavirus (FCoV) of cheetahs, which are...

 

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Genetic analysis of feline caliciviruses associated with a hemorrhagic-like disease (with Mohamed Abd-Eldaim and Leon N.D. Potgieter), Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (2005)

Feline calicivirus (FCV) is 1 of the most common causes of upper respiratory tract disease...

 

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Effect of intranasal vaccination against bovine enteric coronavirus on the occurrence of respiratory tract disease in a commercial backgrounding feedlot (with Paul J. Plummer, Barton W. Rohrbach Dr., R A. Daugherty, K V. Thomas, Rebecca P. Wilkes, and F E. Duggan), Journal of the American Veterinary Medical Association (2004)

Objective - To measure antibody titers against bovine coronavirus (BCV), determine frequency of BCV in...

 

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Detection of feline coronavirus infection in southern African nondomestic felids (with Stephen Kania, E Stylianides, H Bertschinger, D Keet, and M van Vuuren), Journal of wildlife diseases (2003)

Feline coronavirus (FCoV) infects members of the Felidae family with results ranging from seroconversion with...

 

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Detection of feline coronavirus in captive Felidae in the USA (with S Citino, A H. McNabb, A S. Moffatt, K Gertz, and Stephen Kania), Feline coronavirus (FCoV) is an important pathogen of domestic and nondomestic Felidae. Investigation into the prevalence of FCoV in exotic Felidae has relied primarily on serology. The usefulness of genetic detection of FCoV using reverse transcription and nested polymerase chain reaction (RT/nPCR) for viral screening was investigated. Seventy-five biologic samples, primarily feces, from captive felids from 11 institutions were tested using PCR. Serum samples collected from all but 12 of these animals were tested for antibodies to type I and type II FCoV by indirect immunofluorescence. Twenty-four animals were positive using RT/nPCR for virus. Twenty-nine animals were seropositive to type I and/or type II FCoV. From serologic data, infection with a virus antigenically related to FCoV type I occurred most commonly. Serology did not correlate with virus shedding because 13 animals were seronegative to FCoV type I and II but positive using RT/nPCR for virus. Conversely, 20 animals were seropositive but negative using RT/nPCR for FCoV. Some of the populations in which virus was detected had experienced health problems, including feline infectious peritonitis (FIP), necrotizing colitis, and mild enteritis. In addition to its role in FIP, this virus may play a role in gastrointestinal diseases of infected animals. This study demonstrates that FCoV is a significant infectious agent of captive felids because over half of the animals tested were positive by viral genetic detection, serology, or both. Dependence upon one method for detection of infection is unreliable. (2002)

Feline coronavirus (FCoV) is an important pathogen of domestic and nondomestic Felidae. Investigation into the...

 

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Virucidal efficacy of four new disinfectants (with N Z. Elaraky and Leon N.D. Potgieter), Journal of the American Animal Hospital Association (2002)

Virucidal efficacy was evaluated for four recently available disinfectants: chlorine dioxide, potassium peroxymonosulfate, a quaternary...

 

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Deletions in the 7a ORF of feline coronavirus associated with an epidemic of feline infectious peritonitis (with N Boedeker, P Gibbs, and Stephen Kania), Veterinary microbiology (2001)

A population of Persian cats experienced an epidemic of feline infectious peritonitis (FIP) over 2...

 

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Detection of feline coronavirus infection in captive cheetahs (Acinonyx jubatus) by polymerase chain reaction (with S Citino, T Dolorico, A H. McNabb, A S. Moffat, and Stephen Kania), Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians (2001)

Feline coronavirus genetic elements were detected by polymerase chain reaction from blood, fecal samples, and...

 

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Development of an enzyme linked immunosorbent assay (ELISA) for the diagnosis of ophidian paramyxovirus (OPV) (with Stephen A. Kania, N Nowlin, V R. Diderrich, and Edward Ramsay), Reviews of Infectious Diseases (2000)
 

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Novel endotheliotropic herpesviruses fatal for Asian and African elephants (with L K. Richman, R J. Montali, R L. Garber, J Lehnhardt, T Hildebrandt, D Schmitt, D Hardy, D J. Alcendor, and G S. Hayward), Science (New York, N.Y.) (1999)

A highly fatal hemorrhagic disease has been identified in 10 young Asian and African elephants...

 

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Fecal shedding of feline coronavirus in adult cats and kittens in an Abyssinian cattery (with L M. Harplod, Alfred M. Legendre, Paul J. Plummer, K Millsaps, and Barton W. Rohrbach Dr.), Journal of the American Veterinary Medical Association (1999)

15 adult cats and 18 kittens from a single cattery were used to determine patterns...

 

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Correlation of genomic detection of feline coronavirus with various diagnostic assays for feline infectious peritonitis (with K Brenneman, R K. Millsaps, and Leon N.D. Potgieter), Journal of Veterinary Diagnostic Investigation (1998)

Primers for a reverse transcriptase-nested polymerase chain reaction (PCR) for feline coronavirus (FCV) in a...

 

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Serologic reactivity using conserved envelope epitopes in feline lentivirus-infected felids (with S A. Kania and Leon N.D. Potgieter), Journal of Veterinary Diagnostic Investigation (1997)

An ELISA based on synthetic peptides identical to lentivirus envelope protein amino acid sequences was...

 

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The isolation, detection, and cross-reactivity of Asian elephant IgG for the development of serological diagnostic tests (with S A. Kania, L K. Richman, R J. Montali, and Leon N.D. Potgieter), Journal of Veterinary Allergy and Clinical Immunology (1997)

Asian elephant ( Elephas maximus) IgG was isolated and purified using a recombinant protein A...

 

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Encephalitis associated with a variant of equine herpesvirus 1 in a Thomson's gazelle ( Gazella thomsonii) (with Edward Ramsay, V Diderrich, L Richman, G P. Allen, and Leon N.D. Potgieter), Journal of Zoo and Wildlife Medicine (1996)

Abstract: In 1994, a 4-year-old male Thomson's gazelle at the Knoxville Zoological Park (USA) died...

 

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Virucidal efficacy of the newer quaternary ammonium compounds (with V S. Mellon, G Caldwell, and Leon N.D. Potgieter), Journal of the American Animal Hospital Association (1995)

The virucidal activity of several disinfectants containing newer generation quaternary ammonium compounds (QACs) as their...

 

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Enteritis associated with a coronavirus-like agent in a rhea ( Rhea americana) chick. (with K A. Brenneman), Journal of Avian Medicine and Surgery (1995)
 

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Cloning, sequencing, and in vitro expression of glycoprotein gp48 of a noncytopathogenic strain of bovine viral diarrhea virus (with I U. Silva-Krott and Leon N.D. Potgieter), Veterinary microbiology (1994)

Total cellular and viral RNA isolated from cells infected with noncytopathic bovine viral diarrhea virus...