The isolation, detection, and cross-reactivity of Asian elephant IgG for the development of serological diagnostic tests
Asian elephant ( Elephas maximus) IgG was isolated and purified using a recombinant protein A and proteins G affinity matrix and DEAE cibacron blue chromatography. Rabbits were inoculated with elephant IgG to produce anti-Asian elephant IgG. Using an ELISA, it was determined that the anti-Asian elephant sera has strong reactivity with Asian elephant IgG and African elephant ( Loxodonta africana ) IgG, moderate reactivity with manatee ( Trichechus manatus latirostris) IgG and weaker reactivity with IgG from hyrax ( Procavia capensis) and black rhinoceros ( Diceros bicornis). Commercially available antisera produced against cattle, deer, and rabbit IgG react weakly with Asian elephant IgG.
S A. Kania, L K. Richman, Melissa A. Kennedy, R J. Montali, and Leon N.D. Potgieter. "The isolation, detection, and cross-reactivity of Asian elephant IgG for the development of serological diagnostic tests" Journal of Veterinary Allergy and Clinical Immunology 5.4 (1997): 125-128.
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