Antimicrobials are added to swine feed or water to boost the daily growth of pigs and reduce animal death rates at swine facilities, thereby enhancing overall production efficiency and increasing profitability. Tylosin is among the most widely used of the macrolide class of veterinary antibiotics by the swine industry. This study is being conducted over multiple years to examine the fate and transport of antibiotic resistant bacteria (ARB) from fields receiving swine manure application to tile drainage systems.