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Article
Distribution of Myosin Attachment Times Predicted from ViscoelasticMechanics of StriatedMuscle
Journal of Biomedicine and Biotechnology (2011)
  • Bradley M. Palmer
  • Yuan Wang
  • Mark S. Miller, University of Massachusetts - Amherst
Abstract

We demonstrate that viscoelastic mechanics of striated muscle, measured as elastic and viscous moduli, emerge directly from the myosin crossbridge attachment time, tatt, also called time-on. The distribution of tatt was modeled using a gamma distribution with shape parameter, p, and scale parameter, β. At 5mM MgATP, β was similar between mouse α-MyHC (16.0 ± 3.7ms) and β-MyHC (17.9 ± 2.0ms), and p was higher (P < 0.05) for β-MyHC (5.6 ± 0.4 no units) compared to α-MyHC (3.2 ± 0.9). At 1mMMgATP, p approached a value of 10 in both isoforms, but β rose only in the β-MyHC (34.8 ± 5.8ms). The estimated mean tatt (i.e., pβ product) was longer in the β-MyHC compared to α-MyHC, and became prolonged in both isoforms as MgATP was reduced as expected. The application of our viscoelastic model to these isoforms and varying MgATP conditions suggest that tatt is better modeled as a gamma distribution due to its representing multiple temporal events occurring within tatt compared to a single exponential distribution which assumes only one temporal event within tatt.

Disciplines
Publication Date
August, 2011
Publisher Statement
doi:10.1155/2011/592343
This work is licensed under a Creative Commons Attribution 4.0 International License.
Citation Information
Bradley M. Palmer, Yuan Wang and Mark S. Miller. "Distribution of Myosin Attachment Times Predicted from ViscoelasticMechanics of StriatedMuscle" Journal of Biomedicine and Biotechnology Vol. 2011 (2011)
Available at: http://works.bepress.com/mark_miller/8/