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Enhanced Adhesion of Pasteurella multocida to Cultured Turkey Peripheral Blood Monocytes
Infection and Immunity (1999)
  • Ingrid M. Pruimbroom, United States Department of Agriculture
  • Richard B. Rimler, United States Department of Agriculture
  • Mark R. Ackermann, United States Department of Agriculture
Abstract

Capsular hyaluronic acid (HA) mediates adhesion of serogroup A strains of Pasteurella multocida to elicited turkey air sac macrophages (TASM). In contrast, freshly isolated turkey peripheral blood monocytes (TPBM) do not bind serogroup A strains. Following culture of TPBM for 6 days in chamber slides, adhesion of the bacteria to TPBM increased gradually. Incubation in chamber slides coated with entactin-collagen IV-laminin (ECL) attachment matrix or exposure to phorbol myristate acetate (PMA) further enhanced the adhesion ofP. multocida to TPBM. Addition of HA, but not Arg-Gly-Asp peptide, to TPBM culture inhibited bacterial adherence similarly to the inhibition previously reported for TASM. Exposure of TPBM to monoclonal antibody directed against HA-binding cell surface proteoglycan (CD44) decreased binding of P. multocida. Collectively, these findings indicate that P. multocida adhesion to TPBM is mediated by capsular HA and can be increased by culture on ECL attachment matrix or PMA exposure. Additionally, the findings suggest that the capsular mucopolysaccharide of serogroup A strains of P. multocida recognizes an isoform of CD44 expressed on cultured TPBM.

Publication Date
March, 1999
Publisher Statement
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Citation Information
Ingrid M. Pruimbroom, Richard B. Rimler and Mark R. Ackermann. "Enhanced Adhesion of Pasteurella multocida to Cultured Turkey Peripheral Blood Monocytes" Infection and Immunity Vol. 67 Iss. 3 (1999)
Available at: http://works.bepress.com/mark_ackermann/89/