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Article
Nucleation of Iron Oxide Nanoparticles Mediated by Mms6 Protein in Situ
ACS Nano
  • Sanjay Kashyap, Ames Laboratory
  • Taylor J. Woehl, Ames Laboratory
  • Xunpei Liu, Iowa State University
  • Surya K. Mallapragada, Iowa State University
  • Tanya Prozorov, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-2014
DOI
10.1021/nn502551y
Abstract

Biomineralization proteins are widely used as templating agents in biomimetic synthesis of a variety of organic-inorganic nanostructures. However, the role of the protein in controlling the nucleation and growth of biomimetic particles is not well understood, because the mechanism of the bioinspired reaction is often deduced from ex situ analysis of the resultant nanoscale mineral phase. Here we report the direct visualization of biomimetic iron oxide nanoparticle nucleation mediated by an acidic bacterial recombinant protein, Mms6, during an in situ reaction induced by the controlled addition of sodium hydroxide to solution-phase Mms6 protein micelles incubated with ferric chloride. Using in situ liquid cell scanning transmission electron microscopy we observe the liquid iron prenucleation phase and nascent amorphous nanoparticles forming preferentially on the surface of protein micelles. Our results provide insight into the early steps of protein-mediated biomimetic nucleation of iron oxide and point to the importance of an extended protein surface during nanoparticle formation.

Comments

Reprinted (adapted) with permission from ACS Nano 8 (2014): 9097, doi: 10.1021/nn502551y. Copyright 2014 American Chemical Society.

Copyright Owner
American Chemical society
Language
en
File Format
application/pdf
Citation Information
Sanjay Kashyap, Taylor J. Woehl, Xunpei Liu, Surya K. Mallapragada, et al.. "Nucleation of Iron Oxide Nanoparticles Mediated by Mms6 Protein in Situ" ACS Nano Vol. 8 Iss. 9 (2014) p. 9097 - 9106
Available at: http://works.bepress.com/mallapragada_surya_k/28/