Identification of a Dispersed MboI Repeat Family in Five Higher Plant Genomes

S. A. Ranade, National Chemical Laboratory
M. D. Lagu, National Chemical Laboratory
S. M. Patankar, National Chemical Laboratory
M. M. Dabak, National Chemical Laboratory
Madhu S. Dhar, University of Tennessee, Knoxville
V. S. Gupta, National Chemical Laboratory
P. K. Ranjekar, National Chemical Laboratory

Abstract

Digestion of nuclear DNAs of five plants, namelyCucurbita maxima (red gourd),Trichosanthes anguina (snake gourd),Cucumis sativus (cucumber),Cajanus cajan (pigeon pea) andPhaseolus vulgaris (french bean) with the restriction endonucleaseMboI yielded discrete size classes with molecular weights in the range of 0.5 to 5 kbp. TheMboI digestion pattern of Cot 0.1 DNA in french bean is comparable with that of total DNA, indicating that these bands represented highly repeated DNA sequences. Cleavage of the DNAs with varying amounts ofMboI indicated the dispersed nature of the repeat families. Southern hybridization studies using french bean highly repetitive DNA as a probe indicated more homology with repeats of pigeon pea and less homology with red gourd, snake gourd and cucumber repeats