Oil-in-water emulsion as a model system to study the growth of E. coli O157:H7 in a heterogenous food system
Oil-in-water emulsions (hexadecane, minimal media [M9], and Tween 20) were used as model system to study the growth of Escherichia coli O157:H7. Stationary-phase cell density decreased as the hexadecane concentration was increased (0%, 5%, 20%, and 40% [w/v]) and biphasic growth was observed in 40% emulsions supplemented with 0.4% glucose. Thin aggregate fimbriae (curli) were observed using SEM, and a greater percentage (Ps <0.001) of curli-producing colonies were isolated from 40% emulsions. Heat resistance (55 °C) of emulsion-grown cells was greater than broth-grown cells, indicating that growth and physiology under heterogeneous conditions differ from cells grown in liquid.
Lynne A. McLandsborough and P. Prachaiyo. "Oil-in-water emulsion as a model system to study the growth of E. coli O157:H7 in a heterogenous food system" Journal of food Science 68 (2003): 2950-2958.
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