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A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...
One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...
Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...
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Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....
Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...
To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...
The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...
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Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...
Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...
'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...
Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....
Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...
The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...
Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...
A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...
Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...
Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...
Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...
We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...
In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...
We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol....
F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...
The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...
In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...
The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...
Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between...
We studied the binding of actin to the erythrocyte membrane by a novel application of...
Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...
The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...
Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...
The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...
Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...
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We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...
A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...
Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit...