*Actins

A stable, high capacity, F-actin affinity column (with Y. L. Wang, E. W. Voss Jr., D. Branton, and D. L. Taylor), The Journal of biological chemistry (1982)

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...

 

*Carrier Proteins

Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

*Cell Aggregation

Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae (with H. M. Ingalls and C. M. Goodloe-Holland), Proceedings of the National Academy of Sciences of the United States of America (1986)

Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...

 

*Cytoskeletal Proteins

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

*Endocytosis

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

*Membrane Fusion

Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 

*Membrane Proteins

Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

*Microfilament Proteins

Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

*Molecular Probe Techniques

F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 

*Multigene Family

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

*Naphthalenes

Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

3T3 Cells

Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

Actinin

PDF

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

Actins

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 
Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 
Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

PDF

Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 

PDF

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

PDF

Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

PDF

The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

PDF

How actin binds and assembles onto plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1988)

We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol....

 

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity (with V. M. Fowler, J. Swanson, D. Branton, and D. L. Taylor), The Journal of cell biology (1981)

Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between...

 

PDF

Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane (with V. M. Fowler, W. R. Hargreaves, D. L. Taylor, and D. Branton), The Journal of cell biology (1981)

We studied the binding of actin to the erythrocyte membrane by a novel application of...

 

Adaptor Proteins, Signal Transducing

PDF

Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

Adenosine Triphosphate

F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

Alkaloids

Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

Alternative Splicing

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Amino Acid Sequence

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

PDF

Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

Animals

PDF

Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 
Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 
Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 
F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

PDF

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

PDF

Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

PDF

The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 
Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae (with H. M. Ingalls and C. M. Goodloe-Holland), Proceedings of the National Academy of Sciences of the United States of America (1986)

Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...

 
A stable, high capacity, F-actin affinity column (with Y. L. Wang, E. W. Voss Jr., D. Branton, and D. L. Taylor), The Journal of biological chemistry (1982)

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...

 

Aorta

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Arabidopsis

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

Arabidopsis Proteins

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

Base Sequence

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

Binding Sites

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

Binding, Competitive

Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

Biochemistry

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Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

Blood Platelets

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Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

Blood Proteins

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Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

Blotting, Western

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Calcium

Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

PDF

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

Calcium-Binding Proteins

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

Carrier Proteins

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

PDF

Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

PDF

The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

Cattle

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 
Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

Cell Adhesion

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Cytoskeleton--plasma membrane interactions (with A. L. Hitt), Science (New York, N.Y.) (1992)

Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit...

 
Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae (with H. M. Ingalls and C. M. Goodloe-Holland), Proceedings of the National Academy of Sciences of the United States of America (1986)

Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...

 

Cell Aggregation

PDF

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

Cell Communication

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Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

Cell Compartmentation

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

Cell Division

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

Cell Fractionation

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Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 
Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae (with H. M. Ingalls and C. M. Goodloe-Holland), Proceedings of the National Academy of Sciences of the United States of America (1986)

Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...

 

Cell Fusion

Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 

Cell Line

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 
F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 

Cell Membrane

Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 
Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 
Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

PDF

Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

PDF

The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 
Cytoskeleton--plasma membrane interactions (with A. L. Hitt), Science (New York, N.Y.) (1992)

Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit...

 
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

PDF

How actin binds and assembles onto plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1988)

We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol....

 
Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae (with H. M. Ingalls and C. M. Goodloe-Holland), Proceedings of the National Academy of Sciences of the United States of America (1986)

Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity (with V. M. Fowler, J. Swanson, D. Branton, and D. L. Taylor), The Journal of cell biology (1981)

Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between...

 

Cell Movement

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

PDF

Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 
Cytoskeleton--plasma membrane interactions (with A. L. Hitt), Science (New York, N.Y.) (1992)

Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

Cell Physiology

Cytoskeleton--plasma membrane interactions (with A. L. Hitt), Science (New York, N.Y.) (1992)

Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit...

 

Cell Size

PDF

Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 

Cells

Cytoskeleton--plasma membrane interactions (with A. L. Hitt), Science (New York, N.Y.) (1992)

Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit...

 

Cells, Cultured

PDF

Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

Cercopithecus aethiops

PDF

Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 
Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 

Chemistry

PDF

Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane (with V. M. Fowler, W. R. Hargreaves, D. L. Taylor, and D. Branton), The Journal of cell biology (1981)

We studied the binding of actin to the erythrocyte membrane by a novel application of...

 

Chemotaxis

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

PDF

Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 

Chickens

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Cholesterol

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

Chromatography, Affinity

Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 
A stable, high capacity, F-actin affinity column (with Y. L. Wang, E. W. Voss Jr., D. Branton, and D. L. Taylor), The Journal of biological chemistry (1982)

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...

 

Chromatography, Gel

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Chromosome Mapping

Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

Chromosomes, Human, Pair 10

Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

Chymotrypsin

Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Circular Dichroism

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Cloning, Molecular

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

Concanavalin A

Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

Conserved Sequence

Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

COS Cells

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 

Cross-Linking Reagents

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How actin binds and assembles onto plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1988)

We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol....

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

Cytoplasm

Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

Cytoskeleton

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 
Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 
Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

PDF

Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

PDF

The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 
Cytoskeleton--plasma membrane interactions (with A. L. Hitt), Science (New York, N.Y.) (1992)

Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

Detergents

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 
Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 

PDF

Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

Dictyostelium

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

PDF

Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 

PDF

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

PDF

Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

PDF

The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

PDF

How actin binds and assembles onto plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1988)

We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol....

 

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 
Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae (with H. M. Ingalls and C. M. Goodloe-Holland), Proceedings of the National Academy of Sciences of the United States of America (1986)

Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity (with V. M. Fowler, J. Swanson, D. Branton, and D. L. Taylor), The Journal of cell biology (1981)

Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between...

 

Diglycerides

Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

DNA

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

DNA Primers

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 

DNA, Complementary

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Dogs

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

Dose-Response Relationship, Drug

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Down-Regulation

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

Electrophoresis, Gel, Two-Dimensional

Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

Electrophoresis, Polyacrylamide Gel

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

Endopeptidases

Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Enzyme Activation

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Epithelial Cells

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

Erythrocyte Membrane

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Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane (with V. M. Fowler, W. R. Hargreaves, D. L. Taylor, and D. Branton), The Journal of cell biology (1981)

We studied the binding of actin to the erythrocyte membrane by a novel application of...

 
Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Erythrocytes

Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 

PDF

Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane (with V. M. Fowler, W. R. Hargreaves, D. L. Taylor, and D. Branton), The Journal of cell biology (1981)

We studied the binding of actin to the erythrocyte membrane by a novel application of...

 
Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Escherichia coli

Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

Ferrets

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Focal Adhesions

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

Freeze Fracturing

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Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

Fungal Proteins

PDF

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

PDF

Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

PDF

The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

Gelsolin

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

Gene Expression

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Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 

Gene Expression Regulation, Neoplastic

Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

Gene Expression Regulation, Plant

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

Genomic Library

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

Glucosides

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F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

Glutathione Transferase

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

Glycosylphosphatidylinositols

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

Green Fluorescent Proteins

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

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Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

Homeostasis

Cytoskeleton--plasma membrane interactions (with A. L. Hitt), Science (New York, N.Y.) (1992)

Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit...

 

Humans

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 
Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

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Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 
Cytoskeleton--plasma membrane interactions (with A. L. Hitt), Science (New York, N.Y.) (1992)

Proteins at the boundary between the cytoskeleton and the plasma membrane control cell shape, delimit...

 

PDF

Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane (with V. M. Fowler, W. R. Hargreaves, D. L. Taylor, and D. Branton), The Journal of cell biology (1981)

We studied the binding of actin to the erythrocyte membrane by a novel application of...

 
Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Immunoglobulin G

Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 

Immunologic Techniques

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F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

In Situ Hybridization, Fluorescence

Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

Indicators and Reagents

F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 

Intercellular Junctions

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Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

Intermediate Filaments

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Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

Iodine Radioisotopes

F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 

Isoelectric Focusing

Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

Isoenzymes

Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

Isomerism

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Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 

Kidney

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Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

Kinetics

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

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Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

Lamins

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Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 

Lipid Bilayers

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

Luminescent Proteins

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

Macromolecular Substances

Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

Membrane Glycoproteins

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

Membrane Lipids

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A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity (with V. M. Fowler, J. Swanson, D. Branton, and D. L. Taylor), The Journal of cell biology (1981)

Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between...

 

Membrane Microdomains

Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 

Membrane Proteins

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

PDF

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 
Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae (with H. M. Ingalls and C. M. Goodloe-Holland), Proceedings of the National Academy of Sciences of the United States of America (1986)

Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity (with V. M. Fowler, J. Swanson, D. Branton, and D. L. Taylor), The Journal of cell biology (1981)

Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between...

 

PDF

Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane (with V. M. Fowler, W. R. Hargreaves, D. L. Taylor, and D. Branton), The Journal of cell biology (1981)

We studied the binding of actin to the erythrocyte membrane by a novel application of...

 

Mice

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

Microfilament Proteins

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

PDF

Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

PDF

Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

PDF

The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

PDF

F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

Microfilaments

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

PDF

How actin binds and assembles onto plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1988)

We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol....

 

Microscopy, Electron

Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 
Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae (with H. M. Ingalls and C. M. Goodloe-Holland), Proceedings of the National Academy of Sciences of the United States of America (1986)

Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 
A stable, high capacity, F-actin affinity column (with Y. L. Wang, E. W. Voss Jr., D. Branton, and D. L. Taylor), The Journal of biological chemistry (1982)

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...

 

Microscopy, Fluorescence

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Microtubule-Associated Proteins

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

Mitogen-Activated Protein Kinase 1

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Mitogen-Activated Protein Kinase 3

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Models, Biological

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

How actin binds and assembles onto plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1988)

We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol....

 

Models, Genetic

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Models, Molecular

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Molecular Sequence Data

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

PDF

Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

Molecular Weight

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F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 
Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae (with H. M. Ingalls and C. M. Goodloe-Holland), Proceedings of the National Academy of Sciences of the United States of America (1986)

Regions of plasma membrane involved in Dictyostelium discoideum intercellular adhesion resist solubilization with the nonionic...

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads (with C. M. Goodloe-Holland and H. M. Ingalls), The Journal of cell biology (1984)

In novel, low-speed sedimentation assays, highly purified, sonicated Dictyostelium discoideum plasma membrane fragments bind to...

 
Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Multigene Family

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

Muscle Proteins

A stable, high capacity, F-actin affinity column (with Y. L. Wang, E. W. Voss Jr., D. Branton, and D. L. Taylor), The Journal of biological chemistry (1982)

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...

 

Muscle, Skeletal

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 

Muscle, Smooth

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Muscles

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 
A stable, high capacity, F-actin affinity column (with Y. L. Wang, E. W. Voss Jr., D. Branton, and D. L. Taylor), The Journal of biological chemistry (1982)

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...

 

Mutagenesis, Site-Directed

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

Mutation

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Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 

PDF

Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

Myocytes, Smooth Muscle

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

Myosin Subfragments

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A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

Myosin Type II

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

Myosins

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

PDF

A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 

Neoplasm Proteins

Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

Neurofibromin 2

Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

Neuropeptides

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

Neutrophils

Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

Newcastle disease virus

Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 

Nonmuscle Myosin Type IIB

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

Nuclear Localization Signals

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Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

Nuclear Proteins

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 

Oligonucleotides, Antisense

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Peptide Elongation Factors

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Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

Peptide Fragments

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A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments (with C. M. Goodloe-Holland), The Journal of cell biology (1984)

The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads...

 
Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Peptides

Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Phagocytosis

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Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

Phalloidine

F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 

PDF

Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

Phenotype

PDF

Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 

Phosphatidylinositol 4,5-Diphosphate

Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

PDF

Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

Phosphatidylinositols

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

PDF

Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

Phospholipase C

Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

Phospholipase C gamma

Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

Phospholipid Transfer Proteins

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

Phosphoproteins

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

Phosphoric Monoester Hydrolases

Membrane cytoskeleton: PIP(2) pulls the strings (with Thomas Nebl and Sang W. Oh), Current biology : CB (2000)

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels...

 

Phosphorylation

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

PDF

Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

Pinocytosis

PDF

Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium (with A. L. Hitt and J. H. Hartwig), The Journal of cell biology (1994)

Interactions between the plasma membrane and underlying actin-based cortex have been implicated in membrane organization...

 

Plant Roots

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

Plasmids

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Polycyclic Compounds

Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

Polyethylene Glycols

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A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity (with V. M. Fowler, J. Swanson, D. Branton, and D. L. Taylor), The Journal of cell biology (1981)

Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between...

 

Polymerase Chain Reaction

File

Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

Polymers

PDF

How actin binds and assembles onto plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1988)

We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol....

 

Protein Binding

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 
Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 
F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 

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Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

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The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 

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Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 

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F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

Protein Conformation

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 
Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

Protein Folding

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Protein Kinase C

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

Protein Structure, Secondary

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Ponticulin is an atypical membrane protein (with A. L. Hitt and T. H. Lu), The Journal of cell biology (1994)

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and...

 

Protein Structure, Tertiary

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Proteins

F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

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Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

Proteomics

Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 

Protozoan Proteins

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Association of the Dictyostelium 30 kDa actin bundling protein with contact regions (with M. Fechheimer, H. M. Ingalls, and R. Furukawa), Journal of cell science (1994)

'Contact regions' are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium...

 

Pseudopodia

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Ponticulin plays a role in the positional stabilization of pseudopods (with D. C. Shutt, D. Wessels, K. Wagenknecht, A. Chandrasekhar, A. L. Hitt, and D. R. Soll), The Journal of cell biology (1995)

Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma...

 

Quaternary Ammonium Compounds

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Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

Rabbits

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

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Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 
Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 

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Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 
A stable, high capacity, F-actin affinity column (with Y. L. Wang, E. W. Voss Jr., D. Branton, and D. L. Taylor), The Journal of biological chemistry (1982)

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...

 

rac GTP-Binding Proteins

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

rac1 GTP-Binding Protein

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

Radioligand Assay

Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein (with C. P. Chia and A. L. Hitt), Cell motility and the cytoskeleton (1991)

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins...

 

Rats

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

Recombinant Fusion Proteins

F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 
Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

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Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

Recombinant Proteins

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Regulatory Sequences, Nucleic Acid

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

Restriction Mapping

Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

Reverse Transcriptase Polymerase Chain Reaction

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Rhodamines

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Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

RNA, Messenger

Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

Sarcolemma

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Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton (with Sang W. Oh, Robert K. Pope, Kelly P. Smith, Jessica L. Crowley, Thomas Nebl, and Jeanne B. Lawrence), Journal of cell science (2003)

The membrane skeleton protein supervillin binds tightly to both F-actin and membranes and can potentiate...

 

Sepharose

A stable, high capacity, F-actin affinity column (with Y. L. Wang, E. W. Voss Jr., D. Branton, and D. L. Taylor), The Journal of biological chemistry (1982)

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...

 

Sequence Alignment

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

Sequence Analysis, DNA

Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

Sequence Deletion

Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

Sequence Homology

Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

PDF

Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes (with K. Pestonjamasp, M. R. Amieva, C. P. Strassel, W. M. Nauseef, and H. Furthmayr), Molecular biology of the cell (1995)

Actin-binding proteins in bovine neutrophil plasma membranes were identified using blot overlays with 125I-labeled F-actin....

 

Sequence Homology, Amino Acid

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

PDF

Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 
Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

PDF

Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

Signal Transduction

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
Mutant Rac1B expression in Dictyostelium: effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (with S. J. Palmieri, Thomas Nebl, Robert K. Pope, D. J. Seastone, E. Lee, E. H. Hinchcliffe, Greenfield Sluder, D. Knecht, and J. Cardelli), Cell motility and the cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the...

 

Software

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Solubility

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F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes (with L. J. Wuestehube), The Journal of cell biology (1987)

F-actin affinity chromatography and immunological techniques are used to identify actin-binding proteins in purified Dictyostelium...

 

Spectrin

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Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane (with V. M. Fowler, W. R. Hargreaves, D. L. Taylor, and D. Branton), The Journal of cell biology (1981)

We studied the binding of actin to the erythrocyte membrane by a novel application of...

 
Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Spectrometry, Fluorescence

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Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein (with A. Shariff), The Journal of cell biology (1990)

In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin...

 
A stable, high capacity, F-actin affinity column (with Y. L. Wang, E. W. Voss Jr., D. Branton, and D. L. Taylor), The Journal of biological chemistry (1982)

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG...

 

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes (with Thomas Nebl, Kersi N. Pestonjamasp, John D. Leszyk, Jessica L. Crowley, and Sang W. Oh), The Journal of biological chemistry (2002)

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures...

 

Staining and Labeling

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Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum (with M. A. Schwartz), The Journal of cell biology (1986)

The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using...

 

Staurosporine

Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

Subcellular Fractions

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 

Succinimides

F-actin blot overlays, Methods in enzymology (1998)

No abstract provided.

 

Temperature

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

PDF

Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane (with V. M. Fowler, W. R. Hargreaves, D. L. Taylor, and D. Branton), The Journal of cell biology (1981)

We studied the binding of actin to the erythrocyte membrane by a novel application of...

 

Tetradecanoylphorbol Acetate

Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

Threonine

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Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides (with F. Nakamura, L. Huang, Kersi N. Pestonjamasp, and H. Furthmayr), Molecular biology of the cell (1999)

Activation of human platelets with thrombin transiently increases phosphorylation at (558)threonine of moesin as determined...

 

Time Factors

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes (with A. Shariff), Science (New York, N.Y.) (1992)

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence...

 

Tissue Distribution

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Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily (with Kersi N. Pestonjamasp, Robert K. Pope, and J. D. Wulfkuhle), The Journal of cell biology (1998)

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here...

 

Tobacco

Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides (with T. Kaye Peterman, Yamini M. Ohol, and Lisa J. McReynolds), Plant physiology (2004)

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a...

 

Transcription Factors

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Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (with Norio Takizawa, Tara C. Smith, Thomas Nebl, Jessica L. Crowley, Stephen J. Palmieri, Lawrence M. Lifshitz, Anka G. Ehrhardt, Laura M. Hoffman, and Mary C. Beckerle), The Journal of cell biology (2006)

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that...

 

Transfection

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 
Merlin differs from moesin in binding to F-actin and in its intra- and intermolecular interactions (with L. Huang, E. Ichimaru, Kersi N. Pestonjamasp, X. Cui, H. Nakamura, G. Y. Lo, F. I. Lin, and H. Furthmayr), Biochemical and biophysical research communications (1998)

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to...

 

Trypsin

Identification by peptide analysis of the spectrin-binding protein in human erythrocytes (with G. H. Kidd and D. Branton), The Journal of biological chemistry (1979)

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to...

 

Tumor Cells, Cultured

Cloning, characterization, and chromosomal localization of human superillin (SVIL) (with Robert K. Pope, Kersi N. Pestonjamasp, K. P. Smith, J. D. Wulfkuhle, C. P. Strassel, and Jeanne B. Lawrence), Genomics (1998)

Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is...

 

Two-Hybrid System Techniques

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Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle (with Samudra S. Gangopadhyay, Norio Takizawa, Cynthia Gallant, Amy L. Barber, Hyun-Dong Je, Tara C. Smith, and Kathleen G. Morgan), Journal of cell science (2004)

The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility...

 
F-actin and myosin II binding domains in supervillin (with Yu Chen, Norio Takizawa, Jessica L. Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, and Mitsuo Ikebe), The Journal of biological chemistry (2003)

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts....

 

Ultracentrifugation

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The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly (with C. P. Chia, A. Shariff, and S. A. Savage), The Journal of cell biology (1993)

Ponticulin, an F-actin binding transmembrane glycoprotein in Dictyostelium plasma membranes, was isolated by detergent extraction...

 

Ultraviolet Rays

Villin-type headpiece domains show a wide range of F-actin-binding affinities (with D. Vardar, A. H. Chishti, B. S. Frank, A. A. Noegel, Sang W. Oh, M. Schleicher, and C. J. McKnight), Cell motility and the cytoskeleton (2002)

The villin-type "headpiece" domain is a modular motif found at the extreme C-terminus of larger...

 

Vinculin

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Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals (with J. D. Wulfkuhle, I. E. Donina, N. H. Stark, Robert K. Pope, Kersi N. Pestonjamasp, and M. L. Niswonger), Journal of cell science (1999)

A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions,...

 

Viral Fusion Proteins

Role of the cytoplasmic domain of the Newcastle disease virus fusion protein in association with lipid rafts (with V. Dolganiuc, Lori McGinnes, and Trudy G. Morrison), Journal of virology (2003)

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich...

 

Viscosity

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A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity (with V. M. Fowler, J. Swanson, D. Branton, and D. L. Taylor), The Journal of cell biology (1981)

Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between...

 

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Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane (with V. M. Fowler, W. R. Hargreaves, D. L. Taylor, and D. Branton), The Journal of cell biology (1981)

We studied the binding of actin to the erythrocyte membrane by a novel application of...