Early life adversity influences later health and may be mediated by psychosocial, affective, and physiological stress and immune factors. We report evidence from MIDUS biomarker project (N = 845, age 34-84, M = 55.09, SD = 11.70) confirming the predicted relationship between early adversity and frequency of diagnosed chronic illnesses (M = 2.8, SD =1.2). Specific psychosocial and physiological variables were tested as mediators. Lifetime (M = .95, SD = 1.5) and daily (M = 12.8, SD = 1.2) discrimination, urinary cortisol (M = 1.1, SD = 1.2 ug/dL), IL-6 (M =2.8, SD = 2.8 pg/mL) (all ps<.001), and affect solidarity (M = 3.4, SD = .29; p<.05) all mediated the observed relationship. Higher discrimination and IL-6 and lower uCORT were associated with greater chronic illnesses. Higher affect solidarity was associated with fewer chronic illnesses. Early adversity can influence chronic illnesses; however, disease trajectories are open to diverse mediating factors.