Analysis of Vaginal Lactobacilli from Healthy and Infected Brazilian Women

Kingsley C. Anukam, University of Western Ontario
Rafael C.R. Martinez, Departamento de Ana´lises Clı´nicas, Toxicolo´gicas e Bromatolo´gicas, Faculdade de Cieˆncias Farmaceˆuticas de Ribeira˜o Preto,
Sı´lvio A. Franceschini, Sistema Integrado de Sau´de da Universidade de Sa˜o Paulo, Ribeira˜o Preto, Brazil
Maristela C. Patta, Departamento de Ginecologia e Obstetrı´cia, Faculdade de Medicina de Ribeira˜o Preto, Universidade de Sa˜o Paulo, Ribeira˜o Preto,
Silvana M. Quintana, Departamento de Ginecologia e Obstetrı´cia, Faculdade de Medicina de Ribeira˜o Preto, Universidade de Sa˜o Paulo, Ribeira˜o Preto,
Alvaro C. Nunes, Departamento de Biologia Geral, Instituto de Cieˆncias Biolo´gicas, Universidade Federal de Minas Gerais, Belo Horizonte,
Joa˜o L.S. Moreira, Departamento de Biologia Geral, Instituto de Cieˆncias Biolo´gicas, Universidade Federal de Minas Gerais, Belo Horizonte,
Gregor Reid, Canadian Research & Development Centre for Probiotics, Lawson Health Research Institute, Departments of Microbiology and Immunology, and Surgery, University of Western Ontario, London, Ontario, Canada

Abstract

Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H2O2-producing lactobacilli were not associated with protection against VVC.