Articles

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A Genetic System for Clostridium ljungdahlii: a Chassis for Autotrophic Production of Biocommodities and a Model Homoacetogen (with Ching Leang, Toshiyuki Ueki, and Derek R. Lovley), Applied and Environmental Microbiology (2012)

Methods for genetic manipulation of Clostridium ljungdahlii are of interest because of the potential for...

 

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Improved cathode materials for microbial electrosynthesis (with Tian Zhang, Huarong Nie, Timothy S. Bain, Haiyun Lu, Mengmeng Cui, Oona L. Snoeyenbos-West, Ashley E. Franks, Thomas P. Russell, and Derek R. Lovley), Energy and Environmental Science (2012)

Microbial electrosynthesis is a promising strategy for the microbial conversion of carbon dioxide to transportation...

 

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Anaerobic Benzene Oxidation by Geobacter Species (with Tian Zhang, Timothy S. Bain, Melissa A. Barlett, and Derek R. Lovley), Applied and Environmental Microbiology (2012)

The abundance of Geobacter species in contaminated aquifers in which benzene is anaerobically degraded has...

 

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Interspecies electron transfer via H2 and formate rather than direct electrical connections in co-cultures of Pelobacter carbinolicus and Geobacter sulfurreducens (with Amelia-Elena Rotaru, Pravin M. Shrestha, Fanghua Liu, Toshiyuki Ueki, Zarath M. Summers, and Derek R. Lovley), Applied and Environmental Microbiology (2012)

Direct interspecies electron transfer (DIET) is an alternative to interspecies H2/formate transfer as a mechanism...

 

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Promoting direct interspecies electron transfer with activated carbon (with Fanghua Liu, Amelia-Elena Rotaru, Pravin M. Shrestha, Nikhil S. Malvankar, and Derek R. Lovley), Energy and Environmental Science (2012)

Granular activated carbon (GAC) is added to methanogenic digesters to enhance conversion of wastes to...

 

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Electrical Conductivity in a Mixed-Species Biofilm (with Nikhil S. Malvankar, Joanne Lau, Ashley E. Franks, Mark T. Tuominen, and Derek R. Lovley), Applied and Environmental Microbiology (2012)

Geobacter sulfurreducens can form electrically conductive biofilms, but the potential for conductivity through mixed species...

 

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A c-type Cytochrome and a Transcriptional Regulator Responsible for Enhanced Extracellular Electron Transfer in Geobacter Sulfurreducens Revealed by Adaptive Evolution (with Derek Lovley, Pier-Luc Trembaly, Zarath M. Summers, Richard H. Glaven, Karsten Zengler, Christian L. Barrett, Yu Qiu, and Bernhard Ø. Palsson), Environmental Microbiology (2011)

The stimulation of subsurface microbial metabolism often associated with engineered bioremediation of groundwater contaminants presents...

 

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A Shift in the Current: New Applications and Concepts for Microbe-Electrode Electron Exchange (with Derek Lovley), Current Opinion in Biotechnology (2011)

Perceived applications of microbe-electrode interactions are shifting from production of electric power to other technologies,...

 

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Electrosynthesis of Organic Compounds from Carbon Dioxide Catalyzed by a Diversity of Acetogenic Microorganisms (with Derek Lovley, Sarah A. Hensley, Ashley E. Franks, Zarath M. Summers, Jianhong Ou, Trevor L. Woodard, and Oona L. Snoeyenbos-West), Applied and Environmental Microbiology (2011)

Microbial electrosynthesis, a process in which microorganisms use electrons derived from electrodes to reduce carbon...

 

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Gene Expression and Deletion Analysis of Mechanisms for Electron Transfer from Electrodes to Geobacter Sulfurreducens (with Derek Lovley, Sarah M. Stycharz, Richard H. Glaven, Maddalena V. Coppi, Sarah M. Gannon, Lorrie A. Perpetua, and Anna Liu), Bioelectrochemistry (2011)

Geobacter sulfurreducens is one of the few microorganisms available in pure culture known to directly...

 

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Specified Localization of the c-type Cytochrome OmcZ at the Anode Surface in Current-Producing Biofilms of Geobacter Sulfurreducens (with Derek Lovley, Kengo Inoue, Ching Leang, Ashley E. Franks, and Trevor L. Woodard), Environmental Microbiology Reports (2011)

The outer-surface, c-type cytochrome OmcZ is essential for optimal current production with Geobacter sulfurreducens, a...

 

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Tunable Metallic-Like Conductivity in Microbial Nanowire Networks (with Derek Lovley, Nihil S. Malvankar, Madeline Vargas, Ashley E. Franks, Ching Leang, Byoung-Chan Kim, Kengo Inoue, Tunde Mester, Sean F. Covalla, Jessica P. Johnson, Sincent M. Rotello, and Mark T. Tuominen), Nature Nanotechnology (2011)

Electronic nanostructures made from natural amino acids are attractive because of their relatively low cost,...

 

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Microbial Fuel Cells, A Current Review (with Ashley E. Franks), Energies (2010)

Microbial fuel cells (MFCs) are devices that can use bacterial metabolism to produce an electrical...

 

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Electrode-Based Approach for Monitoring in Situ Microbial Activity During Subsurface Bioremediation (with Derek Lovley, Kenneth H. Williams, Ashley E. Franks, Andreas Englert, and Philip E. Long), Environmental Sciences & Technology (2010)

Current production by microorganisms colonizing subsurface electrodes and its relationship to substrate availability and microbial...

 

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Microbial Electrosynthesis:Feeding Microbes Electricity to Convert carbon Dioxide and Water to Multicarbon Extracellular Organic Compounds (with Derek Lovley, Trevor L. Woodward, Ashley E. Franks, and Zarath M. Summers), mBio (2010)

The possibility of providing the acetogenic microorganism Sporomusa ovata with electrons delivered directly to the...

 

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Microtoming Coupled to Microarray Analysis to Evaluate the Spatial Metabolic Status of Geobacter Sulfurreducens Biofilms (with Derek Lovley, Ashley E. Franks, and Richard H. Glaven), ISME Journal (2010)

Further insight into the metabolic status of cells within anode biofilms is essential for understanding...

 

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Molecular Analysis of Phosphate Limitation in Geobacteraceae During the Bioremediation of a Uranium-Contaminated Aquifer (with Derek Lovley, A. Lucie N'Guessan, Hila Elifantz, Paula J. Mouser, Barbara Methѐ, Trevor L. Woodard, Kimberly Manley, Kenneth H. Williams, Michael J. Wilkins, Joern T. Larsen, and Philip E. Long), ISME Journal (2010)

Nutrient limitation is an environmental stress that may reduce the effectiveness of bioremediation strategies, especially...

 

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Reductive Dechlorination of 2-Chlorophenol by Anaeromyxobacter Dehalogenana with Electrode Serving as the Electron Donor (with Derek Lovley, Sarah M. Strycharz, Sarah M. Gannon, Amber R. Boles, and Ashley E. Franks), Environmental Microbiology Reports (2010)
 

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Stimulating the Anaerobic Degradation of Aromatic Hydrocarbons in Contaminated Sediments by Providing an Electron Acceptor (with Tian Zhang, Sarah M. Gannon, Ashley E. Franks, and Derek Lovley), Environmental Microbiology (2010)

The possibility that electrodes might serve as an electron acceptor to simulate the degradation of...

 

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Anode Biofilm Transcriptomics Reveals Outer Surface Components Essential for High Density Current Production in Geobacter Sulfurreducens Fuel Cells (with Derek Lovley, Byoung-Chan Kim, Richard H. Glaven, Jessica P. Johnson, Trevor L. Woodard, Barbara A. Methѐ, Raymond J. DiDonato Jr., Sean F. Covalla, Ashley E. Franks, and Anna Liu), PLoS ONE (2009)

The mechanisms by which Geobacter sulfurreducens transfers electrons through relatively thick (>50 µm) biofilms to...

 

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Cyclic Voltammetry of Biofilms of Wild Type and Mutant Geobacter Sulfurreducens on Fuel Cell Anodes Indicates Possible Roles of OmcB, OmcZ, type IV Pili, and Protons in Extracellular Electron Transfer (with Derek Lovley, Hanno Richter, Hongfei Jia, Daniel A. Lowy, and Leonard M. Tender), Energy & Environmental Science (2009)

Geobacteracea are distinct for their ability to reduce insoluble oxidants including minerals and electrodes without...

 

Hieratchical Bayesian Meta-Analysis Models for Cross-Platform Microarray Studies (with Derek Lovley, Erin M. Conlon, Bradley L. Postier, and Barbara A. Methѐ), Journal of Applied Statistics (2009)

The development of new technologies to measure gene expression has been calling for statistical methods...

 

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Novel Strategy for Three-Dimensional Real-Time Imaging of Microbial Fuel Cell Communities: Monitoring the Inhabitory Effects of Proton Accumulation Within the Anode Biofilm (with Derek Lovley, Ashley E. Franks, Hongfei Jia, Mounir Izallalen, and Trevor L. Woodard), Energy and Environmental Science (2009)

Harvesting electricity from the environment, organic wastes, or renewable biomass with microbial fuel cells (MFCs)...

 

PilR, a Transcriptional Regulator for Pilin and other Genes Required for Fe(III) Reduction in Geobacter Sulfurreducens (with Derek Lovley, Katy Juárez, Byoung-Chan Kim, Leticia Olvera, Gemma Reguera, and Barbara A. Methé), Journal of Molecular Microbiology and Biotechnology (2009)

Growth using Fe(III) as a terminal electron acceptor is a critical physiological process in Geobacter...

 

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Polar Lipid Fatty Acids, LPS-Hydroxy Fatty Acids, and Respiratory Quinones of Three Geobacter Strains, and Variation with Electron Acceptor (with Derek Lovley, David B. Hedrick, Aaron D. Peacock, Trevor L. Woodard, Philip E. Long, and David C. White), Journal of Industrial Microbiology and Biotechnology (2009)

The polar lipid fatty acids, lipopolysaccharide hydroxy-fatty acids, and respiratory quinones of Geobacter metallireducens str....

 

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Selection of a Variant of Geobacter Sulfurreducens with Enhanced Capacity for Current Production in Microbial Fuel Cells (with Derek Lovley, Hana Yia, Byoung-Chan Kim, Ashley E. Franks, Anna Klimes, and Leonard M. Tender), Biosens Bioelectron (2009)

Geobacter sulfurreducens produces current densities in microbial fuel cells that are among the highest known...

 

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Graphite Electrode as a Sole Electron Donor for Reductive Dechlorination of Tetrachlorethene by Geobacter Lovleyi (with Sarah M. Strycharz, Trevor L. Woodard, Jessica P. Johnson, Robert A. Stanford, Frank E. Lӧffler, and Derek Lovley), Applied and Environmental Microbiology (2008)

The possibility that graphite electrodes can serve as the direct electron donor for microbially catalyzed...

 

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Benefits of In-Situ Synthesized Microarrays for Analysis of Gene Expression in Understudied Microorganisms (with Bradley Postier, Raymond DiDonato Jr, Anna Liu, Bryan Frank, Barbara A. Methѐ, and Derek Lovley), Journal of Microbiological Methods (2008)

Although the genome sequences of many microorganisms are now known, whole-genome DNA microarray platforms consisting...

 

Corrigendum to "Insights into Genes Involved in Electricity Generation in Geobacter Sulfurreducens Via Whole Genome Microarray of the OmcF-Deficient Mutant" (with Derek Lovley, Byoung-Chan Kim, Bradley L. Postier, Raymond J. Didonato, and Swades K. Chaudhuri), Bioelectrochemistry (2008)

Geobacter sulfurreducens effectively produces electricity in microbial fuel cells by oxidizing acetate with an electrode...

 

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Electricity Generation by Geobacter Sulfurreducens Attached to Gold Electrodes (with Derek Lovley, Hanno Richter, Kevin McCarthy, Jessica P. Johnson, and Vincent M. Rotello), LangMuir (2008)

The versatility of gold for electrode manufacture suggests that it could be an ideal material...

 

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Gene Transcript Analysis of Assimilatory Iron Limitation in Geobacteraceae During Groundwater Bioremediation (with Regina A. O'Neil, Dawn E. Holmes, Maddalena V. Coppi, Lorrie A. Adams, M Juliana Larrahondo, Joy E. Ward, Trevor L. Woodard, Helen V. Vrionis, A Lucie N'Guessan, and Derek Lovley), Environmental Microbiology (2008)

Limitations on the availability of Fe(III) as an electron acceptor are thought to play an...

 

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Genes for Two Multicopper Proteins Require for Fe(III) Oxide Reduction in Geobacter Sulfurreducens Have different Expression Patterns Both in the Subsurface and on Energy-Harvesting Electrodes (with Derek Lovley, regina A. O'Neil, Tünde Mester, Lorrie A. Perpetua, M Juliana Larrahondo, Richard Glaven, Manju L. Sharma, and Joy E. Ward), Microbiology (2008)

Previous studies have shown that Geobacter sulfurreducens requires the outer-membrane, multicopper protein OmpB for Fe(III)...

 

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Insight Into Genes Involved in Electricity Generation in Geobacter Sulfurreducens Via Whole Genome Microarray Analysis of the OmcF-Deficient Mutant (with Derek Lovley, Byoung-Chan Kim, Bradley L. Postier, Raymond J. DiDonato Jr., and Swades K. Chaudhuri), Bioelectrochemistry (2008)

Geobacter sulfurreducens effectively produces electricity in microbial fuel cells by oxidizing acetate with an electrode...

 

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Power Output and Columbic Efficiencies from Biofilms of Geobacter Sulfurreducens Comparable to Mixed Community Microbial Fuel Cells (with H. Richter, S. F. Covalla, J. P. Johnson, T. L. Woodard, A. L. Orloff, H. Jia, M. Zhang, and Derek Lovley), Environmental Microbiology (2008)

It has been previously noted that mixed communities typically produce more power in microbial fuel...

 

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Subsurface Clade of Geobacteraceae that Predominates in a Diversity of Fe(III)-Reducing Subsurface Environments (with Derek Lovley, Dawn E. Holmes, Regina A. O'Neil, Helen A. Vrionis, Lucie A. N'Guessan, Irene Ortiz-Bernad, Maria J. Larrahondo, Lorrie A. Adams, Joy A. Ward, Julie S. Nicoll, Milind A. Chavan, Jessica P. Johnson, and Philip E. Long), ISME J (2008)

There are distinct differences in the physiology of Geobacter species available in pure culture. Therefore,...

 

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Lack of Electricity Production by Pelobacter Carbinolicus Indicates that the Capacity for Fe(III) Oxide Reduction Does Not Necessarily Confer Electron Transfer Ability to Fuel Cell Anodes (with Hanno Richter, Martin Lanthier, and Derek Lovley), Applied and Environmental Microbiology (2007)

The ability of Pelobacter carbinolicus to oxidize electron donors with electron transfer to the anodes...

 

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Genome-Wide Expression Profilinf in Geobacter Sulfurreducens:Identification of Fur and RposS Transcription Regulatory Sites in a Rel Ssu Mutant (with Derek Lovley, Julia Krushkal, Bin Yan, Laurie N. DiDonato, Marko Puljic, Trevor L. woodard, Ronald M. Adkins, and Barbara A. Methѐ), Functional & Integrative Genomics (2007)

RelGsu is the single Geobacter sulfurreducens homolog of RelA and SpoT proteins found in many...

 

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Geonomic and Microarray Analysis of Aromatics Degradation in Geobacter Metallireducens and Compan to a Geobacter Isolate from a Contaminated Fieldriso Site (with Derek Lovley, Jessica E. Butler, Qiang He, Zhili He, and Jizhong Zhou), BMC Genomics (2007)

Background: Groundwater and subsurface environments contaminated with aromatic compounds can be remediated in situ by...

 

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Involvement of Geobacter Sulfurreducens SfrAB in Acetate Oxidation Rather than Intracellular, Respiration-Linked Fe(III) Citrate Reduction (with Derek Lovley, Maddalena V. Coppi, Regina A. O'Neil, Ching Leang, Franz Kaufmann, Barbara A. Methѐ, Trevor L. Woodard, and Anna Liu), Microbiology (2007)

A soluble ferric reductase, SfrAB, which catalysed the NADPH-dependent reduction of chelated Fe(III), was previously...

 

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Prolixibacter Bellariivorans Gen. Now.,Sp.NOw.,A Sugar-Fermenting, Psychrotolerant Anaerobe of the Phylum Bacteroidetes, Isolated from a Marine-Sediment Fuel Cell (with Derek Lovley, Dawn E. Holmes, Trevor L. Woodard, and Aaron D. Peacock), International Journal of Systematic and Evolutionary Microbiology (2007)

A Gram-negative, non-motile, filamentous, rod-shaped, non-spore-forming bacterium (strain F2T) was isolated from the surface of...

 

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Steady State Protein Levels in Geobacter Metallireducens Grown with Iron (III) Citrate or Nitrate as Terminal Electron Acceptor (with Derek Lovley, Angela J. Ahrendt, Sandra L. Tollaksen, Carl Lindberg, Wenhong Zhu, John R. Yates III, Gyӧrgy Babnigg, and Carol S. Giometti), Proteomics (2007)

Geobacter species predominate in aquatic sediments and submerged soils where organic carbon sources are oxidized...

 

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Role of Red Gsu in stress Response and Fe(III) Reduction in Geobacter Sulfurreducens (with Laurie N. DiDonato, Sara A. Sullivan, Barbara A. Methѐ, Reg England, and Derek Lovley), Journal of Bacteriology (2006)

Geobacter species are key members of the microbial community in many subsurface environments in which...

 

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Biofilm and Nanowire Production Leads to Increased Current in Geobacter Sulfurreducens Fuel Cells (with Gemma Reguera, Julie S. Nicoll, Sean F. Covalla, Trevor L. woodard, and Derek Lovley), Applied and Environmental Microbiology (2006)

Geobacter sulfurreducens developed highly structured, multilayer biofilms on the anode surface of a microbial fuel...

 

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Microarray and Genetics Analysis of Electron Transfer Electrodes in Geobacter Sulfurreducens (with Derek Lovley, Dawn E. Holmes, Swades K. Chaudhuri, Teena Mehta, Barbara A. Methѐ, Anna Liu, Joy E. Ward, Trevor L. Woodard, and Jennifer Webster), Environmental Microbiology (2006)

Whole-genome analysis of gene expression in Geobacter sulfurreducens revealed 474 genes with transcript levels that...

 

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Potential for Quantifying Expression of the Geobacteraceae Citrate Synthase Gene To Assess the Activity of Geobacteraceae in the Subsurface and on Current-Harvesting Electrodes (with Dawn E. Holmes, Regina A. O'Neil, Joy E. Ward, Lorrie A. Adams, Trevor L. Woodard, Helen A. Vrionis, and Derek Lovley), Applied and Environmental Microbiology (2005)

The Geobacteraceae citrate synthase is phylogenetically distinct from those of other prokaryotes and is a...

 

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Adaptation to Disruption of the Electron Transfer Pathway for Fe(III) Reduction in Geobacter Sulfurreducens (with Ching Leang, Lorrie A. Adams, Kuk-Jeong Chin, Barbara A. Methѐ, Jennifer Webster, Manju L. Sharma, and Derek Lovley), Journal of Bacteriology (2005)

Previous studies demonstrated that an outer membrane c-type cytochrome, OmcB, was involved in Fe(III) reduction...

 

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DNA Microarray Analysis of Nitrogen Fixation and Fe(III) Reduction in Geobacter Sulfurreducens (with Jennifer Webster, Barbara A. Methé, Jessica E. Butler, and Derek Lovley), Applied and Environmental Microbiology (2005)

A DNA microarray representing the genome of Geobacter sulfurreducens was constructed for use in global...

 

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Differential Protein Expression in the Metal-Reducing Bacterium Geobacter Sulfurreducens Strain PCA Grown with Fumarate or Ferric Citrate (with Derek Lovley, Tripti Khare, Wenhong Zhu, John R. Yates III, Abraham Esteve-Núñez, and Carol S. Giometti), Proteomics (2005)

Geobacter sulfurreducens, generally considered to be a strict anaerobe, is a predominant microbe in subsurface...

 

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Geobacter Bemifjiensis sp.nov. and Geobacter psychrophilus sp.nov., Two Novel fe(III)-Reducing Subsurface Isolates (with Derek Lovley, Dawn E. Holmes, Trevor L. Woodard, Erich S. Hinlein, and David W. Ostendorf), International Journal of Systematic and Evolutionary Microbiology (2005)

Fe(III)-reducing isolates were recovered from two aquifers in which Fe(III) reduction is known to be...

 

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In Situ Expression of nigD in Geobacteraceae in Subsurface Sediments (with Dawn E. Holmes and Derek Lovley), Applied and Environmental Microbiology (2004)

In order to determine whether the metabolic state of Geobacteraceae involved in bioremediation of subsurface...

 

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Isolation, characterization, and U(VI)-Reducing Potential of a Facultatively Anaerobic, Acid-Resistant Bacterium From Low-pH, Nitrate- and U(VI) Contaminated Subsurface Sediment and Description of Salmonella Subterranea sp.nov. (with Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neil, and Derek Lovley), Applied and Environmental Microbiology (2004)

A facultatively anaerobic, acid-resistant bacterium, designated strain FRCl, was isolated from a low-pH, nitrate- and...

 

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Comparison of 16S rRNA, nofD, recA, gyrB, rpoB and fusA Genes Within the Family Geobacteraceae Fam. Nov. (with Derek Lovley and Dawn E. Holmes), International Journal of Systematic and Evolutionary Microbiology (2004)

The sequences of five conserved genes, in addition to the 16S rRNA gene, were investigated...

 

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Dissimilatory Fe(III) and Mn(IV) Redution (with Derek Lovley and Dawn E. Holmes), Advances in Microbial Physiology (2004)

Dissimilatory Fe(III) and Mn(IV) reduction has an important influence on the geochemistry of modern environments,...

 

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Microorganisms Associated with Uranium Bioremediation in a High Salinity subsurface Sediment (with Kevin T. Finneran and Derek Lovley), Applied and Environmental Microbiology (2003)

Although stimulation of dissimilatory metal reduction to promote the reductive precipitation of uranium has been...

 

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Mechanisms for Accessing Insoluble Fe(III) Oxide During Dissimilatory Fe(III) Reduction by Geothrix Fermentans (with Derek Lovley), Applied and Environmental Microbiology (2002)

Mechanisms for Fe(III) oxide reduction were investigated in Geothrix fermentans, a dissimilatory Fe(III)-reducing microorganism found...

 

Fulvic Acid Oxidation State Detection Using Fluorescence Spectroscopy (with Derek Lovley, Lisa Klapper, Diane M. McKnight, J Robin Fulton, Elizabeth L. Blunt-Harris, and Patrick G. Hatcher), Environmental Sciences & Technology (2002)

Humic substances are a heterogeneous class of moderate molecular weight, yellow-colored biomolecules present in all...

 

Mechanisms for Fe(III) Oxide Reduction in Sedimentary Environments (with Derek Lovley), Geomicrobiol J (2002)

Although it was previously considered that Fe(III)-reducing microorganisms must come into direct contact with Fe(III)...

 

Potential for Bioremediation of Uranium-Contaminated Aquifers with Microbial U(VI) Reduction (with Derek Lovley, Kevin T. Finneran, and Rober T. Anderson), Soil and Sediment Contamination (2002)
 

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Reductive Presipitation of Gold by Dissimilatory Fe(III)-Reducing Bacteria and Archaea (with Kazem Kashefi, Jason M. Tor, and Derek Lovley), Applied and Environmental Microbiology (2001)

Studies with a diversity of hyperthermophilic and mesophilic dissimilatory Fe(III)-reducing Bacteria andArchaea demonstrated that some...

 

Reclassification of Trichlorobacter Thiogenes as Geobacter Thiogenes Comb. Nov. (with Derek Lovley, Dawn E. Holmes, Trevor L. Woodard, and Sean F. Covalla), International Journal of Systematic and Evolutionary Microbiology (2001)

Reclassification of the species Trichlorobacter thiogenes as Geobacter thiogenes comb. nov. is proposed on the...

 

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Lack of Production of Electron-Shuttling Compounds or Solubilization of Fe(III) During Reduction of Insoluble Fe(III) Oxide of Geobacter Metallireducens (with Derek Lovley), Applied and Environmental Microbiology (2000)

Studies with the dissimilatory Fe(III)-reducing microorganism Geobacter metallireducens demonstrated that the common technique of separating...

 

Enrichment of Geobacter Species in Response to Stimulation of Fe(III) Oxide by Geobacter Metallireducens (with Derek Lovley, Robert T. Anderson, and Oona Snoeyenbos-West), Applied and Environmental Microbiology (2000)
 

Role of Prior Exposure on Anaerobic Degradation of Naphthalene and Phenanthrene in Marine Harbor Sediments (with Derek Lovley and Lory A. Hayes), Organic Chemistry (1999)

The anaerobic degradation of the polycyclic aromatic hydrocarbons (PAHs) naphthalene and phenanthrene was investigated in...