Characterization of Developing Bovine Cartilage Using Immuno-SEM

Michelle Gerritsen, Boise State University
Janet Hampikian, Boise State University
Julia Oxford, Boise State University
Megan Frary, Boise State University
Jonathan Henderson, Boise State University

Abstract

Collagen is an important material in tissues of living organisms. Found almost everywhere in the human body, it is important in connective tissues, bone growth, and cartilage. In this work, collagen XI and two of its isoforms, V1b and V2, present in developing cartilage, are investigated using the technique of immuno-SEM. The efficacy of this technique to examine fundamental issues related to ossification is presented. Prior work using alternative techniques has shown that both isoforms were observed in the longitudinal septa, and in a very restricted pericellular pattern in the resting zone. Prior to primary ossification, V1b was detected only in the diaphysis, primarily adjacent to the perichondrium, and not in the epiphysis. The V2-containing isoforms were most strongly expressed in areas of newly forming cartilage, and disappeared as chondrocyte maturation proceeded. The technique of immuno-SEM will yield a better understanding of protein composition and organization within the osteochondral junction.