Articles

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Characterization of Myxococcus xanthus MazF and Implications for a New Point of Regulation (with Tye O. Boynton and Lawrence J. Shimkets), Faculty Publications (2013)

During development, Myxococcus xanthus cells undergo programmed cell death (PCD) whereby 80% of vegetative cells...

 

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Rate, Affinity and Calcium Dependence of Nitric Oxide Synthase Isoform Binding to the Primary Physiological Regulator Calmodulin (with Carol A. Chrestensen, Israel M. Scott, Elijah W. Lee, Aaron M. Rahn, Allan M. Johansen, Brian J. Forsberg, Kyle D. Harris, and John C. Salerno), Faculty Publications (2011)

Using interferometry-based biosensors the binding and release of endothelial and neuronal nitric oxide synthase (eNOS...

 

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Kinetic Characterization of Salmonella FliK-FlhB Interactions Demonstrates Complexity of the Type III Secretion Substrate-Specificity Switch (with Daniel P. Morris, Eric D. Roush, J. Will Thompson, M. Arthur Moseley, and James W. Murphy), Faculty Publications (2010)

The bacterial flagellum is a complex macromolecular machine consisting of more than 20000 proteins, most...

 
A Real Time Optical Biosensor Assay for Amoxicillin in Water Samples (with Israel M. Scott, Lewis J. Kraft, Jonathan D. Parker, Kathryn Daniel, Sarah Kustick, and Diane Kennan), Georgia Journal of Science (2010)
 

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Kinetic Characterization of Salmonella FliK-FlhB Interactions Demonstrates Complexity of the Type III Secretion Substrate-Specificity Switch. (with Morris P. Daniel, Roush D. Eric, Thompson W. J, Moseley M. Arthur, and Murphy W. James), Biochemistry (2010)

The bacterial flagellum is a complex macromolecular machine consisting of more than 20 000 proteins,...

 

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Flagellar Formation in C-Ring-Defective Mutants by Overproduction of FliI, the ATPase Specific for Flagellar Type III Secretion (with Manabu Konishi, Masaomi Kanbe, and Shin-Ichi Aizawa), Journal of Bacteriology (2009)

The flagellar cytoplasmic ring (C ring), which consists of three proteins, FliG, FliM, and FliN,...

 

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The Helicobacter pylori Anti-Sigma Factor FlgM Is Predominantly Cytoplasmic and Cooperates with the Flagellar Basal Body Protein FlhA (with Melanie Rust, Sophie Borchert, Eikie Niehus, Sarah A. Kuehne, Eugenia Gripp, Afrodita Bajceta, Sebastian Suerbaum, Kelly T. Hughes, and Christine Josenhans), Journal of Bacteriology (2009)

Helicobacter pylori requires flagellar motility and orientation to persist actively in its habitat. A particular...

 

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The Helicobacter pylori Anti-Sigma Factor FlgM Is Predominantly Cytoplasmic and Cooperates with the Flagellar Basal Body Protein FlhA (with Melanie Rust, Sophie Borchert, Eike Niehus, Sarah A. Gripp, Afrodita Bajceta, Sebastian Suerbaum, Kelly T. Hughes, and Christine Josenhans), Faculty Publications (2009)

Helicobacter pylori requires flagellar motility and orientation to persist actively in its habitat. A particular...

 

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Hydrodynamic Surface Interactions Enable Escherichia Coli to Seek Efficient Routes to Swim Upstream (with Jane Hill, Ozge Kalkanci, and Yale University), Physical Review Letters (2007)

Escherichia coli in shear flow near a surface are shown to exhibit a steady propensity...

 

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The FliN−FliH Interaction Mediates Localization of Flagellar Export ATPase FliI to the C Ring Complex (with James W. Murphy and Bertha González-Pedrajo), Biochemistry (2006)

FliH regulates the flagellar export ATPase FliI, preventing nonproductive ATP hydrolysis. FliH has been shown...

 

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Analysis of the Cytoplasmic Domains of Salmonella FlhA and Interactions with Components of the Flagellar Export Machinery (with John S. Van Arnham, May Kihara, and Robert M. Macnab), Journal of Bacteriology (2004)

Most flagellar proteins are exported via a type III export apparatus which, in part, consists...

 

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Analysis of an Engineered Salmonella Flagellar Fusion Protein, FliR-FlhB (with John S. Van Arham, May Kihara, and Robert M. Macnab), Journal of Bacteriology (2004)

Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB...

 

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Cannabinoid Receptor-G Protein Interactions: Gαi1-Bound Structures of IC3 and a Mutant with Altered G Protein Specificity (with Amy L. Ulfers, Alexander Miller, Ligong Wang, Debra A. Kendall, and Dale F. Mierke), Protein Science (2002)

The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid...

 

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Structure of the Third Intracellular Loop of the Human Cannabinoid 1 Receptor (with Amy L. Ulfers, Debra A. Kendall, and Dale F. Mierke), Biochemistry (2002)

The third cytoplasmic loop (IC3) is a determinant in the dynamic life cycle of G...

 

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An Artificial Transmembrane Segment Directs SecA, SecB, and Electrochemical Potential-dependent Translocation of a Long Amino-terminal Tail (with Debra A. Kendall), The Journal of Biological Chemistry (1999)

Many integral membrane proteins contain an amino-terminal segment, often referred to as an N-tail, that...

 

Popular Press

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NIH Regional Grant-Writing Seminar Wrap-Up (with Daniella Tapu), KSU Funding Forum (2007)

The authors describe a National Institutes of Health (NIH)-sponsored grant writing seminar they attended, aiming...

 

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BD Talon Resin Does Not Bind E. coli SlyD, a Common Contaminant in Ni-NTA IMAC (with Robert M. Macnab), Clontechniques (2004)

E. coli SlyD, a prolyl isomerase, specifically binds divalent metal ions, which can result in...

 

Presentations

Investigation of the Dynamics of the Interactions Among FliN, FliH and FliI of the Salmonella enterica Flagellar Export Apparatus. Poster/National (with JoLeanna Wilson and Israel M. Scott), Gordon Research Conference on Sensory Transduction in Microorganisms (2010)
 
Development of a Real Time Optical Biosensor Assay for Amoxicillin in Complex Samples. Poster/National (with Israel M. Scott and Lewis J. Kraft), Annual Biomedical Research Conference for Minority Students (2009)
 
Dynamic Interactions Among Salmonella enterica Flagellar Export Apparatus Proteins: A Kinetic Analysis. Poster/Regional (with JoLeanna Wilson, Jonathan D. Parker, and Israel M. Scott), 95th Annual American Society for Microbiology Southeastern Branch Conference (2009)
 
Kinetic Survey of Interactions Among Proteins of the Salmonella Flagellar Type III Secretion Apparatus. Poster/Regional (with Jonathan D. Parker and Israel M. Scott), Northeast Regional Meeting of the American Chemical Society (2009)

While many interactions among proteins of the flagellar Type III secretion (T3S) export apparatus have...

 
A Competition Binding Assay for Quantitation of Digoxin Using Biolayer Interferometry. Poster/Local (with John K. Staples and Lewis J. Kraft), Science 2009 Unplugged (2009)
 
Kinetic Survey of Salmonella enterica Flagellar Export Apparatus Protein-Protein Interactions. Poster/International (with Israel M. Scott), 3rd ASM Conference on Salmonella: Biology, Pathogenesis & Prevention (2009)
 
Forensic Applications of Immunological Methods. Invited/Local, Kennesaw State University CHEM 3800 (2009)
 
Development of A Real Time Optical Biosensor Assay for B-lactam Contamination in Waste and Drinking Water. Poster/Local (with Israel M. Scott and Lewis J. Kraft), Herty Medal Undergraduate Research Symposium (2009)
 
Development of a Competitve Binding Assay for Quantitation of Digoxin Using Biolayer Interferometry. Paper/Regional (with Lewis J. Kraft and John K. Staples), 60th Southeastern Regional Meeting of the American Chemical Society (SERMACS) (2008)

Biolayer interferometry (BLI) is an emerging technology for analyzing biomolecular interactions. Similar to surface plasmon...

 
Identification of Cyclized Intermediate Demonstrates Autocatalytic Cleavage of Salmonella FlhB, 60th Southeastern Regional Meeting of the American Chemical Society (SERMACS) (2008)

Salmonella enterica flagellar export apparatus protein FlhB plays a critical role in the substrate specificity...

 
See One, Do One, Teach One: A Medical Training Paradigm Extended to Undergraduate Biochemistry Laboratories. Poster/National (with Carol A. Chrestensen), 20th Biennial Conference on Chemical Education (2008)

Medical educators have long incorporated a “see one, do one, teach one”(“SODOTO”) model of learning...

 
The Flagellar C Ring Component FliN is not Essential for Flagellar Assembly but for Mobility. Poster/National (with Masaomi Kanbe and Shin-Ichi Aizawa), American Society for Microbiology 108th General Meeting (2008)
 
Investigation of the Mechanism of FlhB Cleavage. Poster/National (with Natalie M. Kyriacou), 235th ACS National Meeting (2008)
 
Organization of the Flagellar Export Apparatus: Analysis of a FliO-FliP Fusion. Poster/National (with Martha Ivett Kyriacou), 235th ACS National Meeting (2008)
 
Determination of Affinity Constants for Binding of Salmonella Flagellar Export Proteins FliH and FliI. Poster/National (with Viet Q. Nguyen and Lewis J. Kraft), 2008 American Society for Biochemistry and Molecular Biology Meeting (2008)

Interaction of Salmonella enterica FliH with FliI is critically important to flagellar assembly, a Type...

 
Development of a Non-Isotopic Saturation Binding Assay for Use in the Investigation of Flagellar Export Dynamics. Poster/International (with Lewis J. Kraft and Viet Q. Nguyen), 2008 American Society for Biochemistry and Molecular Biology Meeting (2008)

FliI is the flagellar export ATPase in Salmonella enterica. It is negatively regulated by FliH,...

 
Development of a Non-Isotopic Saturation Binding Assay for Use in the Investigation of Flagellar Export Dynamics. Poster/Local (with Lewis J. Kraft and Viet Q. Nguyen), First Academia/Industry Forum, Georgia Section of the ACS (2008)
 
Dynamic Interactions Among Salmonella Flagellar Export Apparatus Proteins. Poster/International (with Lewis J. Kraft, Viet Q. Nguyen, M. Ivett Kyriacou, and Natalie M. Kyriacou), Gordon Research Conference on Sensory Transduction in Microorganisms (2008)
 
Organization of the membrane-bound export apparatus of Salmonella. Poster/Local (with M. Ivett Kyriacou and Natalie M. Kyriacou), First Academia/Industry Forum, Georgia Section of the ACS (2008)
 
The Flagellar C Ring Component FliN is not Essential for Flagellar Assembly but for Mobility. Poster/National (with Masaomi Kanbe and Shin-Ichi Aizawa), Gordon Research Conference on Sensory Transduction in Microorganisms (2008)
 
Preparing for the MCAT and, More Importantly, Medical School. Invited/Local, KSU American Medical Students Association Chapter (pre-medical) meeting (2007)
 
A fliN Temperature-Sensitive Mutant Manifests in Weaker Binding of FliN to FliH (with James W. Murphy and Bertha González-Pedrajo), Gordon Research Conference on Sensory Transduction In Microorganisms (2006)
 
Flagellar Assembly: Protein Export in a Proton-Driven Nanomachine, Kennesaw State University Department of Chemistry & Biochemistry (2005)
 
The Bacterial Flagellum: Proton-Driven Nanomachine and Protein Export Apparatus, Georgia Southern University Department of Chemistry (2005)
 
The Bacterial Flagellum: Reversible Motor and Type III Protein Export Apparatus, Fisher-Sparks Memorial Lecture at University of Connecticut (2005)
 
FliH is required for FliI binding to the C-ring complex in Salmonella (with Bertha González-Pedrajo, John S. Van Arnam, and Robert M. Macnab), Bacterial Locomotion and Signal Transduction (BLAST) VIII (2005)

FliH regulates the flagellar export ATPase FliI, preventing nonproductive ATP hydrolysis. (FliH)2 and FliI form...

 
Interactions Among the Membrane Proteins of the Flagellar Export Apparatus in Salmonella typhimuium (with John S. Arnam, May Kihara, and Robert M. Macnab), Gordon Research Conference on Sensory Transduction in Microorganisms (2004)
 
SecA-, SecB-, and Electrochemical Potential-Dependent Translocation of a Type III Integral Membrane Protein (with Debra A. Kendall), 1999 ASBMB Meeting (1999)

Abstract published in FASEB Journal, 13(7), A1388.