A method for detecting and counting pollen particles based on Coulter counting principle is presented. This approach also provides information on the size and surface charges of the micro particles, allowing for preliminary differentiation of pollens from other micro particles. Three samples are studied: polymethyl methacrylate particles, tree pollens from Juniperus Scopulorum and grass pollens from Secale Cerale. The samples, suspended in diluted KCl aqueous solutions in an electrochemical cell, were allowed to pass through a microchannel and the conductance of the microchannel was sampled with a Gamry®Potentiostat. The changes in the conductance due to the passing of the micro particles was thus recorded and analyzed. The experimental results showed that tree pollens and grass pollens display distinctive behaviors. The phenomena may be attributed to the differences in the surface characteristics of the pollens and is potentially useful for counting and differentiating different micro particles.