The overarching goal of this project is to understand how chronic acidification and nitrogen enrichment of watersheds influences coupled biogeochemical cycling in streams. Embedded in the project were two primary research elements: 1) examining nitrogen satuartion and the extent of coupling between nitrogen and phosphorus cycling and 2) resolving the interactions among acidification, phosphorus bioavailability and biotic demand for nitrogen and phosphorus. The research involved a series of stable isotope tracer experiments to document nitrogen uptake under ambient and elevated phosphrous conditions and examination of a suite of key microbial processes (denitrification, decomposition, microbial enzyme activity) at two whole-watershed experiment sites. A microcosm experiment was used to examine the influence of acidity stress on animal and microbial stoichiometry.