Articles

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Molecular dynamics analysis of apolipoprotein-D - lipid hydroperoxide interactions: mechanism for selective oxidation of Met-93 (with Aaron J. Oakley, Surabhi Bhatia, and Brett Garner), Faculty of Science - Papers (2012)

Background: Recent studies suggest reduction of radical-propagating fatty acid hydroperoxides to inert hydroxides by interaction...

 
Binding of the molecular chaperone alphaB-crystallin to Abeta amyloid fibrils inhibits fibril elongation (with S Wang, Tuomas P. Knowles, C M Dobson, Mark E. Welland, Christopher A. Waudby, S Meehan, A K Buell, S L Shammas, and J A Carver), Faculty of Science - Papers (2011)

The molecular chaperone ¿B-crystallin is a small heat-shock protein that is upregulated in response to...

 
Enhanced molecular chaperone activity of the small heat-shock protein alphaB-cystallin following covalent immobilization onto a solid-phase support (with V Bellotti, J A. Carver, H J Griesser, B Thierry, J G Shapter, S S Griesser, S Giorgetti, M R Nussio, J A Gerrard, and J Garvey), Faculty of Science - Papers (2011)

The well-characterized small heat-shock protein, alphaB-crystallin, acts as a molecular chaperone by interacting with unfolding...

 
Monotremes provide a key to understanding the evolutionary significance of epididymal sperm maturation (with Brett Nixon, Russell C. Jones, and Jean-Louis Dacheux), Faculty of Science - Papers (2011)

"It has been widely accepted that mammalian spermatozoa are infertile when they leave the testes...

 
NMR spectroscopy of 14-3-3zeta reveals a flexible C-terminal extension: differentiation of the chaperone and phosphoserine-binding activities of 14-3-3zeta (with H Fu, Danielle M. Williams, John A. Carver, Lixin Zhang, Huanqin Dai, Joanna M. Woodcock, and K L. Goodwin), Faculty of Science - Papers (2011)

Intracellular 14-3-3 proteins bind to many proteins, via a specific phosphoserine motif, regulating diverse cellular...

 

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AlphaB-crystallin inhibits the cell toxicity associated with amyloid fibril formation by kappa-casein and the amyloid-beta peptide (with Francis C. Dehle, Ian F. Musgrave, and John A. Carver), Faculty of Science - Papers (2010)

Amyloid fibril formation is associated with diseases such as Alzheimer’s, Parkinson’s, and prion diseases. Inhibition...

 

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Carboxymethylated-k-casein: A convenient tool for the identification of polyphenolic inhibitors of amyloid fibril formation (with John A. Carver, Peter J. Duggan, Yanqin Liu, Adam G. Meyer, and C E. Tranberg), Faculty of Science - Papers (2010)

Reduced and carboxymethylated-κ-casein (RCM-κ-CN) is a milk-derived amyloidogenic protein that readily undergoes nucleation-dependent aggregation and...

 

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Small heat-shock proteins interact with a flanking domain to suppress polyglutamine aggregation (with Amy L. Roberston, Stephen J. Headey, Helen M. Saunders, Martin J. Scanlon, John A. Carver, and Stephen P. Bottomley), Faculty of Science - Papers (2010)

Small heat-shock proteins (sHsps) are molecular chaperones that play an important protective role against cellular...

 

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The dissociated form of kappa-casein is the precursor to its amyloid fibril formation (with John Carver, Yanqin Liu, and David Thorn), Faculty of Science - Papers (2010)

Bovine milk kappa-casein forms a self-associating oligomeric micelle-like species, in equilibrium with dissociated forms. In...

 

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The interaction of alphaB-crystallin with mature alpha-synuclein amyloid fibrils inhibits their elongation (with Christopher A. Waudby, Tuomas P. J Knowles, Glyn L. Devlin, Jeremy N. Skepper, John A. Carver, Mark E. Welland, John Christodoulou, Christopher M. Dobson, and Sarah Meehan), Faculty of Science - Papers (2010)

alphaB-Crystallin is a small heat-shock protein (sHsp) that is colocalized with alpha-synuclein (alphaSyn) in Lewy...

 

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The interaction of unfolding α-lactalbumin and malate dehydrogenase with the molecular chaperone αB-crystallin: a light and X-ray scattering investigation (with J W. Regini, Sarah Meehan, Kristen Bremmell, Matthew J. Clarke, Donna Lammie, Tim Wess, and John A. Carver), Faculty of Science - Papers (2010)

Purpose: The molecular chaperone αB-crystallin is found in high concentrations in the lens and is...

 

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Crystallin proteins and amyloid fibrils (with John A. Carver), Faculty of Science - Papers (2009)

Improper protein folding (misfolding) can lead to the formation of disordered (amorphous) or ordered (amyloid...

 

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(-)-Epigallocatechin-3-gallate (EGCG) maintains k-casein in its pre-fibrillar state without redirecting its aggregation pathway (with Sean A. Hudson, Francis C. Dehle, Ian F. Musgrave, and John Carver), Faculty of Science - Papers (2009)

The polyphenol (-)-epigallocatechin-3-gallate (EGCG) has recently attracted much research interest in the field of protein-misfolding...

 

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Model for amorphous aggregation processes (with Samual Stranks, Steve Van Sluyter, Elizabeth J. Waters, John A. Carver, and Lorenz von Smekal), Faculty of Science - Papers (2009)

The amorphous aggregation of proteins is associated with many phenomena, ranging from the formation of...

 

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New proteins identified in epididymal fluid from the platypus (Ornithorhynchus anatinus) (with Jean-Louis Dacheux, Francoise Dacheux, Valerie Labas, Brett Nixon, and Russell C. Jones), Faculty of Science - Papers (2009)

The platypus epididymal proteome is being studied because epididymal proteins are essential for male fertility...

 
Protective interactions of dairy peptides with fibril structures and relevance to Alzheimer's disease (with Louise Bennett, Williams Roderick, Yanqin Liu, Sunanda Sudharmarajan, and John A. Carver), Faculty of Science - Papers (2009)

Selected dairy caseins have been shown to have capacity for chaperone-like regulation of folding pathways...

 

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Testicular descent, sperm maturation and capacitation. Lessons from our most distant relatives, the monotremes (with Russell C. Jones, Jean-Louis Dacheux, and Brett Nixon), Faculty of Science - Papers (2009)

The present review examines whether monotremes may help to resolve three questions relating to sperm...

 

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The thioflavin T fluorescence assay for amyloid fibril detection can be biased by the presence of exogenous compounds (with Sean A. Hudson, Tak W. Kee, and John A. Carver), Faculty of Science - Papers (2009)

Thioflavin T (ThT) dye fluorescence is used regularly to quantify the formation and inhibition of...

 

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The two faced nature of milk casein proteins: amyloid fibril formation and chaperone-like activity (with David Thorn and John A. Carver), Faculty of Science - Papers (2009)

Molecular chaperones are a diverse group of proteins that stabilise partially folded target proteins to...

 
Amyloid fibril formation by bovine milk alpha(s2)-casein occurs under physiological conditions yet is prevented by its natural counterpart, alpha(s1)-casein (with David Thorn, M Sunde, Stephen Poon, and John A. Carver), Faculty of Science - Papers (2008)

The calcified proteinaceous deposits, or corpora amylacea, of bovine mammary tissue often comprise a network...

 

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Dissociation from the oligomeric state is the rate-limiting step in fibril formation by kappa-casein (with Tomas Koudelka, David Thorn, Danielle M. Williams, Glyn Devlin, Peter Hoffmann, and John A. Carver), Faculty of Science - Papers (2008)

Amyloid fibrils are aggregated and precipitated forms of protein in which the protein exists in...

 
The effect of small molecules in modulating the chaperone activity of alpha B-crystallin against ordered and disordered protein aggregation (with John A. Carver), Faculty of Science - Papers (2008)

Protein aggregation can proceed via disordered or ordered mechanisms, with the latter being associated with...

 

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Unraveling the mysteries of protein folding and misfolding (with John A. Carver), Faculty of Science - Papers (2008)

This mini-review focuses on the processes and consequences of protein folding and misfolding. The latter...

 

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Site-directed mutations in the C-terminal extension of human αB-crystallin affect chaperone function and block amyloid fibril formation (with T. M. Treweek, D. M. Williams, S. Meehan, J. A. Carver, and M. J. Walker), Faculty of Health and Behavioural Sciences - Papers (2007)

Alzheimer’s, Parkinson’s and Creutzfeldt-Jakob disease are associated with inappropriate protein deposition and ordered amyloid fibril...

 
Characterisation of amyloid fibril formation by small heat-shock proteins, human alphaA-, alphaB- and R120G alphaB-crystallins (with Sarah Meehan, Tuomas P. Knowles, Andrew J. Baldwin, Jeffrey F. Smith, Adam M. Squires, Phillip Clements, Teresa M. Treweek, Gian Tartaglia, Michele Vendruscolo, Cait MacPhee, Christopher Dobson, and John Carver), Graduate School of Medicine - Papers (2007)

AlphaB-Crystallin is a ubiquitous small heat-shock protein (sHsp) renowned for its chaperone ability to prevent...

 

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Mimicking phosphorylation of alphaB-crystallin affects its chaperone activity (with Sarah Meehan, J Horwitz, Andrew Aquilina, J L Benesch, C V Robinson, Cait MacPhee, and John Carver), Faculty of Science - Papers (2007)
 
Role of the epididymis in sperm competition (with Russell C. Jones, JL Dacheux, and Brett Nixon), Faculty of Science - Papers (2007)

Although it is generally understood that the testes recruited kidney ducts for reproductive function during...

 

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An epididymal form of cauxin, a carboxylesterase-like enzyme, is present and active in mammalian male reproductive fluids (with Maya Belghazi, JL Dacheux, M Miyazaki, T Yamashita, and JL Gatti), Faculty of Science - Papers (2006)

Mass spectrometric analysis of a prion protein (PrP)-containing complex isolated from ram cauda epididymal fluid...

 

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The epididymal soluble prion protein forms a high-molecular-mass complex in association with hydrophobic proteins (with Maya Belghazi, Jean-Louis Dacheux, and Jean-Luc Gatti), Faculty of Science - Papers (2005)

We have shown previously that a 'soluble' form of PrP (prion protein), not associated with...

 

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Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation (with Mark Baker, Louise Hethrington, Shaun D. Roman, and Robert J. Aitken), Faculty of Science - Papers (2004)

The capacitation of mammalian spermatozoa involves the activation of a cAMP-mediated signal transduction pathway that...

 

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Compartmentalization of prion isoforms within the reproductive tract of the ram (with Pierre Sarradin, Jean-Louis Dacheux, and Jean-Luc Gatti), Faculty of Science - Papers (2004)

Cellular prion protein (Prp(C)) is a glycoprotein usually associated with membranes via its glycosylphosphatidylinositol (GPI)...

 
Post-testicular sperm environment and fertility (with Jean-Luc Gatti, Sandrine Castella, Francoise Dacheux, S Metayer, Veronique Thimon, and Jean-Louis Dacheux), Faculty of Science - Papers (2004)

When mammalian spermatozoa exit the testis, they show a highly specialized morphology; however, they are...

 

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The development of signal transduction pathways during epididymal maturation is calcium dependent (with Kelly Asquith, Russell C. Jones, and Robert J. Aitken), Faculty of Science - Papers (2004)

Capacitation has been correlated with the activation of a cAMP-PKA-dependent signaling pathway leading to protein...

 

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Endogenous redox activity in mouse spermatozoa and its role in regulating the tyrosine phosphorylation events associated with sperm capacitation (with Russell C. Jones and Robert J. Aitken), Faculty of Science - Papers (2003)

We investigated the role of endogenous redox activity in regulating the signal transduction pathway leading...

 

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Tyrosine phosphorylation of HSP-90 during mammalian sperm capacitation (with Russell C. Jones and Robert J. Aitken), Faculty of Science - Papers (2003)

The process of sperm capacitation is correlated with activation of a signal transduction pathway leading...

 

Contributions to Books

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The two-faced nature of small heat shock proteins: amyloid assembly and the inhibition of fibril formation. Relevance to disease states (with S Meehan and John A. Carver), Faculty of Science - Papers (2010)

The ability of small heat-shock proteins (sHsps) such as alphaB-crystallin to inhibit the amorphous (disordered)...

 

Presentations

Is amyloid making your milk go sour? (with David C. Thorn, Teresa M. Treweek, and John Carver), Graduate School of Medicine - Papers (2006)