Optochemical nanosensors for intracellular chemical measurement

Raoul Kopelman, University of Michigan - Ann Arbor
Michael T. Miller, University of Michigan - Ann Arbor
Murphy Brasuel, University of Michigan - Ann Arbor
Heather Clark, University of Michigan - Ann Arbor
Marion Hoyer, University of Michigan - Ann Arbor
Martin Philbert, University of Michigan - Ann Arbor

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Originally published as: Raoul Kopelman, Michael T. Miller, Murphy Brasuel, Heather A. Clark, Marion Hoyer, and Martin A. Philbert, "Optochemical nanosensors for intracellular chemical measurement," Proc. SPIE 3540, 198 (1999). DOI:10.1117/12.339797

Abstract

The development of a submicron optical fiber "supertip" has provided advantages over previously produced submicron tips, such as facilitating insertion of these sensors into cells while minimizing damage to the cell membrane. Fiber optic ion correlation-based nanosensors for sodium, potassium and chloride employing these "supertips" have been applied to the monitoring of ion concentrations in single mouse oocytes. These sensors have also been used to monitor the effect of an ion channel-blocking agent. In order to address the challenge associated with single-cell simultaneous measurement of multiple analytes, the use of submicron optical fiber multiprobes has been explored.