Optochemical nanosensors for intracellular chemical measurement
Originally published as: Raoul Kopelman, Michael T. Miller, Murphy Brasuel, Heather A. Clark, Marion Hoyer, and Martin A. Philbert, "Optochemical nanosensors for intracellular chemical measurement," Proc. SPIE 3540, 198 (1999). DOI:10.1117/12.339797
The development of a submicron optical fiber "supertip" has provided advantages over previously produced submicron tips, such as facilitating insertion of these sensors into cells while minimizing damage to the cell membrane. Fiber optic ion correlation-based nanosensors for sodium, potassium and chloride employing these "supertips" have been applied to the monitoring of ion concentrations in single mouse oocytes. These sensors have also been used to monitor the effect of an ion channel-blocking agent. In order to address the challenge associated with single-cell simultaneous measurement of multiple analytes, the use of submicron optical fiber multiprobes has been explored.
Raoul Kopelman, Michael T. Miller, Murphy Brasuel, Heather Clark, Marion Hoyer, and Martin Philbert. "Optochemical nanosensors for intracellular chemical measurement" Bouvé Faculty Publications (1998).
Available at: http://works.bepress.com/hclark/5