Time series data for 3B image processing

Four time series data sets of Stellaris single molecules RNA FISH (fluorescence in situ hybridization). All four datasets are 301 imaqe planes. FISH probes are to TOP1 mRNA (Topoisomerase I)in Saos osterosarcoma cells.

One dataset is 10 millisecond exposures (very dim), acquired in Streaming acquisition mode (no hardware overhead).

The other three datasets are a three consecutive planes of the same XY field of view.

I have included image processing results for:

PiMP - a fast method developed by Sebastian Munck et al 2012, http://www.ncbi.nlm.nih.gov/pubmed/?term=22357945

and greatly improved performance by Glen Macdonald (Seattle).

3B microscopy - Susan Cox and Ed Rosten

3B = Bayesian analysis of blinking and bleaching.

http://www.ncbi.nlm.nih.gov/pubmed/23361088

http://www.ncbi.nlm.nih.gov/pubmed/22138825

http://www.coxphysics.com/3b/

http://www.superresolved.com/

The 3B result is of a small field of view with date/time "stamps" as part of the filenames.