This contribution aims to investigate preliminary toxicity; antioxidant and some pharmacological activities include

analgesic and anti-inflammatory activity of polyphenol-rich fractions of Ximenia americana L. The toxicological pattern was studied by the determination of LD50 in mice. The antioxidant potential of the sample was evaluated using three separate methods, hydroxyl radical scavenging assay; hydrogen peroxide scavenging assay and phosphomolybdate assay for the total antioxidant capacity. The analgesic activity used two separate methods such as the test of writhing induced by acetic acid and hot pot test. The anti-inflammatory profile was

evaluated using induction in the right-hand paw by injection of formalin. Polyphenol-rich fractions from Ximenia americana L., exhibit maximum radical scavenging activity. Concerning preliminary toxicity study; LD50 is 3270.8 mg/Kg body weight for intraperitoneal administration. It could say that extracts of roots are no toxic.Fraction exhibited significant analgesic and anti-inflammatory activity in doses of 200, 250 and 300 mg/kg

b. wt. and particularly higher dosage level (300 mg/kg b. wt) induced significant anti-inflammatory and analgesic activities. The results indicated that the polyphenol-rich fractions of Ximenia americana L., possessed antioxidant, antiinflammatory and analgesic properties, and may give credence to some of its ethnopharmacological uses. We undertook this study of roots from this plant in order to provide a scientific basis for the traditional use of Ximenia americana L., in traditional medicine. Our results of this study appeared to show the safety of acute toxicity of extract from of Ximenia americana L., which can, therefore, be continuously used with safety in traditional medicine.