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Article
Genome-Wide Gene Regulation of Biosynthesis and Energy Generation by a Novel Transcriptional Repressor in Geobacter Species
Nucleic Acids Research (2010)
  • Derek Lovley, University of Massachusetts - Amherst
  • Toshiyuki Ueki
Abstract

Geobacter species play important roles in bioremediation of contaminated environments and in electricity production from waste organic matter in microbial fuel cells. To better understand physiology of Geobacter species, expression and function of citrate synthase, a key enzyme in the TCA cycle that is important for organic acid oxidation in Geobacter species, was investigated. Geobacter sulfurreducens did not require citrate synthase for growth with hydrogen as the electron donor and fumarate as the electron acceptor. Expression of the citrate synthase gene, gltA, was repressed by a transcription factor under this growth condition. Functional and comparative genomics approaches, coupled with genetic and biochemical assays, identified a novel transcription factor termed HgtR that acts as a repressor for gltA. Further analysis revealed that HgtR is a global regulator for genes involved in biosynthesis and energy generation in Geobacter species. The hgtR gene was essential for growth with hydrogen, during which hgtR expression was induced. These findings provide important new insights into the mechanisms by which Geobacter species regulate their central metabolism under different environmental conditions.

Disciplines
Publication Date
2010
Publisher Statement
This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Nucleic Acids Research following peer review. The definitive publisher-authenticated version Nucl. Acids Res. (2010) 38(3): 810-821 doi:10.1093/nar/gkp1085 is available online at: http://nar.oxfordjournals.org/content/38/3/810.full OPEN ACCESS
Citation Information
Derek Lovley and Toshiyuki Ueki. "Genome-Wide Gene Regulation of Biosynthesis and Energy Generation by a Novel Transcriptional Repressor in Geobacter Species" Nucleic Acids Research Vol. 38 Iss. 3 (2010)
Available at: http://works.bepress.com/derek_lovley/47/