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Crystallization of a FE,ZN Superoxide-Dismutase from the Archaebacterium Thermoplasma-Acidophilium

D.C. Morris
D.G. Searcy
B.F.P. Edwards

Abstract

The novel Fe,Zn superoxide dismutase from the archaebacterium Thermoplasma acidophilium has been crystallized in space groups P1, P2(1) and P2(1)2(1)2, with 2,4 and 1/2 of an 84,000 Mr tetramer, respectively, estimated to be in the asymmetric unit of the unit cell. The orthorhombic crystals, which have unit cell dimensions a = 84.2 A, b = 72.7 A, c = 67.8 A, diffract X-rays to at least 2.0 A and are suitable for a determination of the three-dimensional structure of the Fe,Zn superoxide dismutase.

Suggested Citation

D.C. Morris, D.G. Searcy, and B.F.P. Edwards. "Crystallization of a FE,ZN Superoxide-Dismutase from the Archaebacterium Thermoplasma-Acidophilium" Journal of Molecular Biology 186.1 (1985): 213-214.
Available at: http://works.bepress.com/dennis_searcy/23



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