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Insecticide resistance in house flies from the United States: Resistance levels and frequency of pyrethroid resistance alleles

Jeffrey G. Scott, Cornell University
Cheryl A. Leichter, Cornell University
Frank D. Rinkevihc, Cornell University
Sarah A. Harris, Cornell University
Cathy Su, Cornell University
Lauren C. Aberegg, Cornell University
Roger Moon, University of Minnesota - Twin Cities
Christopher J. Geden, USDA-ARS CMAVE
Alec C. Gerry, University of California - Riverside
David B. Taylor, USDA-ARS Agroecosystem Management Research Unit
Ronnie L. Byford, New Mexico State University - Main Campus
Wes Watson, North Carolina State University at Raleigh
Gregory Johnson, Montana State University - Billings
David Boxler, University of Nebraska-Lincoln
Ludek Zurek, Kansas State University

Abstract

Although insecticide resistance is a widespread problem for most insect pests, frequently the assessment of resistance occurs over a limited geographic range. Herein, we report the first widespread survey of insecticide resistance in the USA ever undertaken for the house fly, Musca domestica, a major pest in animal production facilities. The levels of resistance to six different insecticides were determined (using discriminating concentration bioassays) in 10 collections of house flies from dairies in nine different states. In addition, the frequencies of Vssc and CYP6D1 alleles that confer resistance to pyrethroid insecticides were determined for each fly population. Levels of resistance to the six insecticides varied among states and insecticides. Resistance to permethrin was highest overall and most consistent across the states. Resistance to methomyl was relatively consistent, with 65–91% survival in nine of the ten collections. In contrast, resistance to cyfluthrin and pyrethrins + piperonyl butoxide varied considerably (2.9–76% survival). Resistance to imidacloprid was overall modest and showed no signs of increasing relative to collections made in 2004, despite increasing use of this insecticide. The frequency of Vssc alleles that confer pyrethroid resistance was variable between locations. The highest frequencies of kdr, kdr-his and super-kdr were found in Minnesota, North Carolina and Kansas, respectively. In contrast, the New Mexico population had the highest frequency (0.67) of the susceptible allele. The implications of these results to resistance management and to the understanding of the evolution of insecticide resistance are discussed.

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