Article
Effect of Genome-Plastome Interaction on Meiosis and Pollen Development in Oenothera Species And Hybrids
Sexual Plant Reproduction
(1997)
Abstract
Oenothera villaricae Dietrich and Oe. picensis Dietrich, complete translocation heterozygotes, are fully interfertile, giving rise to six discrete classes of true breeding hybrids from a reciprocal cross. Associated with each parent and hybrid is a characteristic ab01tive non staining pollen fraction easily distinguished from fully developed pollen under the light microscope. Pollen abortion has been associated with translocation rings in other angiosperm species, and may characterize such sys tems. The abortive pollen fraction is significantly differ ent between reciprocal Oenothera hybrids, however (P<0.001), indicative of partial cytoplasmic control. Pol len abortion is most severe in the F 1 hybrid generation, and ameliorates with successive generations of hybrid self-fertilization. Three-way analysis of variance shows significant effects on pollen stainability (a measure of the non-abortive fraction) for nucleus, cytoplasm and selfed hybrid generation, individually or in combinations. This result suggests a combined nucleocytoplasmic basis for the pollen abortion. Correlated with the observation of in creased pollen abortion in Oenothera hybrids are meiotic findings of broken chromosome rings (chains, univa lents), asymmetric anaphase chromosome distributions and trinucleate tetrads. To test the hypothesis that such anomalous meiotic events play a role in the mechanism of pollen abortion, meiotic disjunction frequency was de termined for each parent, F1 and F9 selfed hybrid acces sions. Three-way analysis of variance shows levels of sig nificance comparable to those noted for pollen stainabili ty (P
Keywords
- Translocation rings,
- Nondisjunction,
- Microsporogenesis,
- Oenothera
Disciplines
Publication Date
1997
Publisher Statement
Sexual Plant Reproduction had a title change to Plant Reproduction in 2012
Citation Information
Michael J. Chapman and David L. Mulcahy. "Effect of Genome-Plastome Interaction on Meiosis and Pollen Development in Oenothera Species And Hybrids" Sexual Plant Reproduction Vol. 10 (1997) Available at: http://works.bepress.com/david_mulcahy/1/