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Genetic Evidence of a Long-Range RNA-RNA Interaction between the Genomic 5' -Untranslated Region and Nonstructural Protein 1 Coding Region in Murine and Bovine Coronaviruses. (with B. J. Guan, Y. P. Su, and H. Y. Wu), Journal of Virology (2012)

Higher-order RNA structures in the 5'-untranslated regions (UTRs) of the mouse hepatitis coronavirus (MHV) and...

 

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An optimal cis-replication stem-loop IV in the 5' untranslated region of the mouse coronavirus genome extends 16 nucleotides into open reading frame 1. (with B. J. Guan and H. Y. Wu), Journal of Virology (2011)

The 288-nucleotide (nt) 3' untranslated region (UTR) in the genome of the bovine coronavirus (BCoV)...

 

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Subgenomic messenger RNA amplification in coronaviruses. (with H. Y. Wu), Proceedings of the National Academy of Sciences (2010)

Coronaviruses possess the largest known RNA genome, a 27- to 32-kb (+)-strand molecule that replicates...

 

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Bovine coronavirus nonstructural protein 1 (p28) is an RNA binding protein that binds terminal genomic cis-replication elements. (with K. M. Gustin, B. J. Guan, and A. Dziduszko), Journal of Virology (2009)

Nonstructural protein 1 (nsp1), a 28-kDa protein in the bovine coronavirus (BCoV) and closely related...

 

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5'-proximal hot spot for an inducible positive-to-negative-strand template switch by coronavirus RNA-dependent RNA polymerase. (with H. Y. Wu), 5'-proximal hot spot for an inducible positive-to-negative-strand template switch by coronavirus RNA-dependent RNA polymerase. (2007)

Coronaviruses have a positive-strand RNA genome and replicate through the use of a 3' nested...

 

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An RNA stem-loop within the bovine coronavirus nsp1 coding region is a cis-acting element in defective interfering RNA replication. (with C. G. Brown, K. S. Nixon, and S. D. Senanayake), Journal of Virology (2007)

Higher-order cis-acting RNA replication structures have been identified in the 3'- and 5'-terminal untranslated regions...

 

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Bovine coronavirus 5'-proximal genomic acceptor hotspot for discontinuous transcription is 65 nucleotides wide. (with H. Y. Wu and A. Ozdarendeli), Journal of Virology (2006)

Coronaviruses are positive-strand, RNA-dependent RNA polymerase-utilizing viruses that require a polymerase template switch, characterized as...

 

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Coronavirus genome structure and replication. (with R. S. Baric), Current Topics in Microbiology and Immunology (2005)

In addition to the SARS coronavirus (treated separately elsewhere in this volume), the complete genome...

 

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Stem-loop IV in the 5' untranslated region is a cis-acting element in bovine coronavirus defective interfering RNA replication. (with S. Raman), Journal of Virology (2005)

The 210-nucleotide (nt) 5' untranslated region (UTR) in the positive-strand bovine coronavirus (BCoV) genome is...

 

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Common RNA replication signals exist among group 2 coronaviruses: evidence for in vivo recombination between animal and human coronavirus molecules. (with H. Y. Wu, J. S. Guy, D. Yoo, R. Vlasak, and E. Urbach), Virology (2003)

5' and 3' UTR sequences on the coronavirus genome are known to carry cis-acting elements...

 

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Stem-loop III in the 5' untranslated region is a cis-acting element in bovine coronavirus defective interfering RNA replication. (with S. Raman, P. Bouma, and G. D. Williams), Journal of Virology (2003)

Higher-order structures in the 5' untranslated region (UTR) of plus-strand RNA viruses are known in...

 

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Downstream sequences influence the choice between a naturally occurring noncanonical and closely positioned upstream canonical heptameric fusion motif during bovine coronavirus subgenomic mRNA synthesis. (with A. Ozdarendeli, S. Ku, S. Rochat, G. D. Williams, and S. D. Senanayake), Journal of Virology (2001)

Mechanisms leading to subgenomic mRNA (sgmRNA) synthesis in coronaviruses are poorly understood but are known...

 

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Downstream ribosomal entry for translation of coronavirus TGEV gene 3b. (with J. B. O'Connor), Virology (2000)

Gene 3b (ORF 3b) in porcine transmissible gastroenteritis coronavirus (TGEV) encodes a putative nonstructural polypeptide...

 

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A phylogenetically conserved hairpin-type 3' untranslated region pseudoknot functions in coronavirus RNA replication. (with G. D. Williams and R. Y. Chang), Journal of Virology (1999)

Secondary and tertiary structures in the 3' untranslated region (UTR) of plus-strand RNA viruses have...

 

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The major product of porcine transmissible gastroenteritis coronavirus gene 3b is an integral membrane glycoprotein of 31 kDa. (with J. B. O'Connor), Virology (1999)

The open reading frame potentially encoding a polypeptide of 27.7 kDa and located as the...

 

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Translation from the 5' untranslated region (UTR) of mRNA 1 is repressed, but that from the 5' UTR of mRNA 7 is stimulated in coronavirus-infected cells. (with S. D. Senanayake), Journal of Virology (1999)

Viral gene products are generally required in widely differing amounts for successful virus growth and...

 

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Bovine coronavirus I protein synthesis follows ribosomal scanning on the bicistronic N mRNA. (with S. D. Senanayake), Virus Research (1997)

The mRNA encoding the 49-kDa nucleocapsid protein (N) of the bovine coronavirus is bicistronic. A...

 

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Coronavirus genomic and subgenomic minus-strand RNAs copartition in membrane-protected replication complexes. (with P. B. Sethna), Journal of Virology (1997)

The majority of porcine transmissible gastroenteritis coronavirus plus-strand RNAs (genome and subgenomic mRNAs), at the...

 

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cis Requirement for N-specific protein sequence in bovine coronavirus defective interfering RNA replication. (with R. Y. Chang), Journal of Virology (1996)

A naturally occurring 2.2-kb defective interfering (DI) RNA of the bovine coronavirus, structurally a simple...

 

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Tandem placement of a coronavirus promoter results in enhanced mRNA synthesis from the downstream-most initiation site. (with R. Krishnan and R. Y. Chang), Virology (1996)

Insertion of the 17-nucleotide promoter region for the bovine coronavirus N gene as part of...

 

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The UCUAAAC promoter motif is not required for high-frequency leader recombination in bovine coronavirus defective interfering RNA. (with R. Y. Chang and R. Krishnan), Journal of Virology (1996)

The 65-nucleotide leader on the cloned bovine coronavirus defective interfering (DI) RNA, when marked by...

 

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Evidence for a pseudoknot in the 3' untranslated region of the bovine coronavirus genome. (with G. D. Williams and R. Y. Chang), Advances in Experimental Medicine and Biology (1995)

A potential pseudoknot was found in the 3' untranslated region of the bovine coronavirus genome...

 

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Precise large deletions by the PCR-based overlap extension method. (with S. D. Senanayake), Molecular Biotechnology (1995)

The authors describe an efficient method for generating large deletions (> 200 nts) of precise...

 

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A cis-acting function for the coronavirus leader in defective interfering RNA replication. (with R. Y. Chang, M. A. Hoffman, and P. B. Sethna), Journal of Virology (1994)

To test the hypothesis that the 65-nucleotide (nt) leader on subgenomic mRNAs suffices as a...

 

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Role of subgenomic minus-strand RNA in coronavirus replication. (with R. Y. Chang, M. A. Hofmann, and P. B. Sethna), Archives of Virology, Supplement (1994)

Coronavirus subgenomic minus-strand RNAs (negative-strand copies of the 3' coterminal subgenomic mRNAs) probably function in...

 

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A translation-attenuating intraleader open reading frame is selected on coronavirus mRNAs during persistent infection. (with M. A. Hofmann and S. D. Senanayake), Proceedings of the National Academy of Sciences (1993)

Short open reading frames within the 5' leader of some eukaryotic mRNAs are known to...

 

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An intraleader open reading frame is selected from a hypervariable 5' terminus during persistent infection by the bovine coronavirus. (with M. A. Hofmann and S. D. Senanayake), Advances in Experimental Medicine and Biology (1993)
 

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Leader-mRNA junction sequences are unique for each subgenomic mRNA species in the bovine coronavirus and remain so throughout persistent infection. (with M. A. Hofmann, R. Y. Chang, and S. Ku), Virology (1993)

The common leader sequence on bovine coronavirus subgenomic mRNAs and genome was determined. To examine...

 

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Sequencing DNA amplified directly from a bacterial colony. (with M. A. Hofmann), Methods in Molecular Biology (1993)

A few hundred bacterial cells obtained by touching a bacterial colony with a sterile toothpick...

 

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The Coronaviridae now comprises two genera, coronavirus and torovirus: report of the Coronaviridae Study Group. (with D. Cavanagh, M. A. Brinton, L. Enjuanes, K. V. Holmes, M. C. Horzinek, M. M. Lai, H. Laude, P. G. Plagemann, S. G. Siddell, and et al.), Advances in Experimental Medicine and Biology (1993)

At the April 1992, mid-term meeting of the International Committee on Taxonomy of Viruses (ICTV)...

 

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The 9-kDa hydrophobic protein encoded at the 3' end of the porcine transmissible gastroenteritis coronavirus genome is membrane-associated. (with F. Y. Tung, S. Abraham, M. Sethna, and B. G. Hogue), Virology (1992)

The open reading frame potentially encoding a 78 amino acid, 9101 Da hydrophobic protein (HP)...

 

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The nucleocapsid protein gene of bovine coronavirus is bicistronic. (with S. D. Senanayake, M. A. Hofmann, and J. L. Maki), Journal of Virology (1992)

For animal RNA viruses that replicate through an RNA intermediate, reported examples of bicistronic mRNAs...

 

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A PCR-enhanced method for determining the 5' end sequence of mRNAs. (with M. A. Hofmann), PCR Methods and Applications (1991)

A method was developed to amplify ligated cDNA copies of mRNA 5' ends before cloning...

 

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Minus-strand copies of replicating coronavirus mRNAs contain antileaders. (with P. B. Sethna and M. A. Hofmann), Journal of Virology (1991)

The 5' leader sequence on mRNAs of the porcine transmissible gastroenteritis coronavirus was determined and...

 

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Sequencing PCR DNA amplified directly from a bacterial colony. (with M. A. Hofmann), Biotechniques (1991)

We show that PCR product asymmetrically amplified directly from a bacterial colony can be sequenced...

 

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The 5' end of coronavirus minus-strand RNAs contains a short poly(U) tract. (with M. A. Hofmann), Journal of Virology (1991)

A radiolabeled oligodeoxynucleotide primer that anneals near the common 5' end of bovine coronavirus minus-strand...

 

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Bovine coronavirus mRNA replication continues throughout persistent infection in cell culture. (with M. A. Hoffman and P. B. Sethna), Journal of Virology (1990)

The existence of viral mRNA replicons was demonstrated in cells infected with the bovine coronavirus...

 

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Coronavirus subgenomic replicons as a mechanism for mRNA amplification. (with P. B. Sethna and S. L. Hung), Advances in Experimental Medicine and Biology (1990)
 

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Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site. (with S. Abraham, T. E. Kienzle, and W. Lapps), Journal of Virology (1990)

The sequence of the spike (also called peplomer or E2) protein gene of the Mebus...

 

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Recommendations of the Coronavirus Study Group for the nomenclature of the structural proteins, mRNAs, and genes of coronaviruses. (with D. Cavanagh, L. Enjuanes, K. V. Holmes, M. M. Lai, H. Laude, S. G. Siddell, W. Spaan, F. Taguchi, and P. J. Talbot), Virology (1990)
 

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Sequence and expression analysis of potential nonstructural proteins of 4.9, 4.8, 12.7, and 9.5 kDa encoded between the spike and membrane protein genes of the bovine coronavirus. (with S. Abraham, T. E. Kienzle, and W. E. Lapps), Virology (1990)

The nucleotide sequence between the spike and membrane protein genes in the bovine coronavirus (BCV)...

 

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Structure and expression of the bovine coronavirus hemagglutinin protein. (with T. E. Kienzle, S. Abraham, and B. G. Hogue), Advances in Experimental Medicine and Biology (1990)
 

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Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein. (with T. E. Kienzle, S. Abraham, and B. G. Hogue), Journal of Virology (1990)

The sequence of the hemagglutinin-esterase (HE) gene for the Mebus strain of bovine coronavirus was...

 

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Coronavirus subgenomic minus-strand RNAs and the potential for mRNA replicons. (with P. B. Sethna and S. L. Hung), Proceedings of the National Academy of Sciences (1989)

The genome of the porcine transmissible gastroenteritis coronavirus is a plus-strand, polyadenylylated, infectious RNA molecule...

 

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Synthesis and processing of the bovine enteric coronavirus haemagglutinin protein. (with B. G. Hogue and T. E. Kienzle), Journal of General Virology (1989)

The haemagglutinin molecule on the bovine enteric coronavirus has been identified as a glycoprotein of...

 

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Molecular cloning of complementary DNA from a pneumopathic strain of bovine viral diarrhea virus and its diagnostic application. (with K. V. Brock, B. T. Rouse, and L. N. Potgieter), Canadian Journal of Veterinary Research (1988)

A pneumopathic strain of bovine viral diarrhea virus was grown in cell culture and purified....

 

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Temporal regulation of bovine coronavirus RNA synthesis. (with J. G. Keck, B. G. Hogue, and M. M. Lai), Virus Research (1988)

The structure and synthesis of bovine coronavirus (BCV)-specific intracellular RNA were studied. A genome-size RNA...

 

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The amino-terminal signal peptide on the porcine transmissible gastroenteritis coronavirus matrix protein is not an absolute requirement for membrane translocation and glycosylation. (with P. A. Kapke, F. Y. Tung, B. G. Hogue, R. D. Woods, and R. Wesley), Virology (1988)

cDNA clones mapping within the first 2601 bases of the 3' end of the porcine...

 

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Deduced amino acid sequence and potential O-glycosylation sites for the bovine coronavirus matrix protein. (with W. Lapps and B. G. Hogue), Advances in Experimental Medicine and Biology (1987)

The nucleotide sequence of the matrix (M) protein gene of the bovine coronavirus (BCV) was...

 

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Diagnosis of porcine and bovine enteric coronavirus infections using cloned cDNA probes. (with L. J. Shockley, P. A. Kapke, W. Lapps, L. N. Potgieter, and R. Woods), Journal of Clinical Microbiology (1987)

Molecular clones representing the first 2,000 bases from the 3' end of the porcine transmissible...

 

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Glycosylation of the bovine coronavirus hemagglutinin protein. (with B. G. Hogue), Advances in Experimental Medicine and Biology (1987)

The bovine coronavirus hemagglutinin protein gp140 is composed of disulfide-linked subunits of 65 kDa. This...

 

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Nucleotide sequence of the porcine transmissible gastroenteritis coronavirus matrix protein gene. (with P. A. Kapke, F. Y. Tung, R. D. Woods, and R. Wesley), Advances in Experimental Medicine and Biology (1987)

cDNA clones mapping within the first 2601 bases of the 3' end of the TGEV...

 

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Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes. (with W. Lapps and B. G. Hogue), Virology (1987)

The 3' end of the 20-kb genome of the Mebus strain of bovine enteric coronavirus...

 

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Temporal regulation of RNA synthesis of bovine coronavirus. (with J. G. Keck, B. G. Hogue, and M. M. Lai), Advances in Experimental Medicine and Biology (1987)
 

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Sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein gene. (with P. A. Kapke), Virology (1986)

The 3' end of the 20-kb genome of the Purdue strain of porcine transmissible gastroenteritis...

 

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Structural proteins of human respiratory coronavirus OC43. (with B. G. Hogue), Virus Research (1986)

The human respiratory coronavirus OC43 was grown on a human rectal tumor cell line and...

 

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Bovine coronavirus hemagglutinin protein. (with B. King and B. J. Potts), Virus Research (1985)

Treatment of purified bovine coronavirus (Mebus strain) with pronase destroyed the integrity of virion surface...

 

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Coronavirus-like particles and Campylobacter in marmosets with diarrhea and colitis. (with R. G. Russell, A. Lenhard, L. N. Potgieter, D. Gillespie, and N. K. Clapp), Digestive Diseases and Science (1985)
 

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Oligonucleotide fingerprints of antigenically related bovine coronavirus and human coronavirus OC43. (with W. Lapps), Archives of Virology (1985)

Virion RNAs from the bovine enteric coronavirus and the human respiratory coronavirus OC43 were compared...

 

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Antigenic relationships among proteins of bovine coronavirus, human respiratory coronavirus OC43, and mouse hepatitis coronavirus A59. (with B. G. Hogue and B. King), Journal of Virology (1984)

Antisera prepared against each of three single and one pair of major structural proteins of...

 

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Bovine coronavirus structural proteins. (with B. King), Journal of Virology (1982)

The tissue culture-adapted strain (Mebus) of bovine coronavirus was grown in the presence of isotopically...

 

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RNA-dependent RNA polymerase activity in coronavirus- infected cells. (with D. E. Dennis), Journal of Virology (1982)

An enzymatic activity which incorporates [3H]UMP into acid-precipitable material in the presence of endogenous template...

 

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Coronavirus cell-associated RNA-dependent RNA polymerase. (with D. E. Dennis), Advances in Experimental Medicine and Biology (1981)
 

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Genome of porcine transmissible gastroenteritis virus. (with D. E. Dennis and J. S. Guy), Journal of Virology (1980)

The Purdue strain of transmissible gastroenteritis virus, a porcine coronavirus, was grown to titers of...

 

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Bovine coronavirus genome. (with J. S. Guy), Journal of Virology (1979)

The tissue culture-adapted strain (Mebus) of the bovine coronavirus was grown to titers of greater...