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Article
Raman Multiplexers for Alternative Gene Splicing
Analytical Chemistry
(2008)
Abstract
Nonfluorescent labels were used for an array-format multiplex detection of alternative splice junctions of breast cancer susceptibility gene 1 (BRCA1) by surface-enhanced Raman scattering (SERS). A four-plex detection scheme using nonfluorescent labels was demonstrated for DNA sequences specific to four BRCA1 alternative splice variants: Δ(11q) (the last 3309 nt deleted from exon 11), Δ(9, 10) (exon 9 and 10 deleted), Δ(5) (exon 5 deleted), and Δ(5q, 6) (the last 22 nt of exon 5 and the entire exon 6 is deleted). This is the first proof-of-concept study to apply SERS-based detection using nonfluorescent labels to investigate alternative gene splicing. Detection sensitivity of up to 1 fM was demonstrated for the Raman labels chosen to clearly discriminate the splice junctions via specific target identification. The proposed approach has the potential to become a highly sensitive and selective tool for comprehensive alternative splicing profiling of BRCA1 or other genes relevant to specific diseases.
Disciplines
Publication Date
May 1, 2008
Publisher Statement
Posted with permission from Analytical Chemistry 80 (2008): 3342–3349. Copyright 2008 American Chemical Society.
Citation Information
Lan Sun, Chenxu Yu and Joseph Irudayaraj. "Raman Multiplexers for Alternative Gene Splicing" Analytical Chemistry Vol. 80 Iss. 9 (2008) Available at: http://works.bepress.com/chenxu_yu/10/