In silico design for synthesis of molecularly imprinted microspheres specific towards bisphenol A by precipitation polymerization

Chanin Nantasenamat, 1Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University
Chartchalerm Isarankura-Na-Ayudhya, 1Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University
Leif Bülow, Pure and Applied Biochemistry, Chemical Center, Lund University
Lei Ye, Pure and Applied Biochemistry, Chemical Center, Lund University
Virapong Prachayasittikul, 1Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University

Abstract

Bisphenol A (BPA), a ubiquitous chemical used in industries, has attracted great attention due to its widespread leakage to the environment and foodstuff. This has spurred great interest in the preparation of synthetic polymers capable of selectively sequestering BPA. In this study, theoretical calculation was utilized to confirm the selection of suitable functional monomer capable of strong interaction with BPA. It was found that 4-vinylpyridine was the optimal functional monomer as demonstrated in the literature. A series of molecularly imprinted polymers (MIPs) were prepared by varying the functional monomer, cross-linker, and porogen. After template removal, rebinding with the original template molecule was carried out in acetonitrile, acetonitrile/water (50/50 – 20/80, v/v). 4-vinylpyridine co-polymerized with ethylene glycol dimethacrylate (4VPY-co-EDMA) and 4-vinylpyridine co-polymerized with trimethylolpropane trimethacrylate (4VPY-co-TRIM) were found to exhibit good binding performance towards bisphenol A in acetonitrile. However, only 4VPY-co-EDMA was able to maintain its imprinting effect in acetonitrile/water (50/50 v/v) whereas 4VPY-co-TRIM totally lost its imprinting effect.