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Article
Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths
Micromachines
  • Joseph T. Banovetz, Iowa State University
  • Min Li, Iowa State University
  • Darshna Pagariya, Iowa State University
  • Sungu Kim, Iowa State University
  • Baskar Ganapathysubramanian, Iowa State University
  • Robbyn Anand, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
4-23-2019
DOI
10.3390/mi10040271
Abstract

Clusters of biological cells play an important role in normal and disease states, such as in the release of insulin from pancreatic islets and in the enhanced spread of cancer by clusters of circulating tumor cells. We report a method to pattern cells into clusters having sizes correlated to the dimensions of each electrode in an array of wireless bipolar electrodes (BPEs). The cells are captured by dielectrophoresis (DEP), which confers selectivity, and patterns cells without the need for physical barriers or adhesive interactions that can alter cell function. Our findings demonstrate that this approach readily achieves fine control of cell cluster size over a broader range set by other experimental parameters. These parameters include the magnitude of the voltage applied externally to drive capture at the BPE array, the rate of fluid flow, and the time allowed for DEP-based cell capture. Therefore, the reported method is anticipated to allow the influence of cluster size on cell function to be more fully investigated.

Comments

This article is published as Banovetz, Joseph T., Min Li, Darshna Pagariya, Sungu Kim, Baskar Ganapathysubramanian, and Robbyn K. Anand. "Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths." Micromachines 10, no. 4 (2019): 271. DOI: 10.3390/mi10040271. Posted with permission.

Creative Commons License
Creative Commons Attribution 4.0 International
Copyright Owner
The Authors
Language
en
File Format
application/pdf
Citation Information
Joseph T. Banovetz, Min Li, Darshna Pagariya, Sungu Kim, et al.. "Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths" Micromachines Vol. 10 Iss. 4 (2019) p. 271
Available at: http://works.bepress.com/baskar-ganapathysubramanian/84/