In this article, the influence of bolt holes, specifically their number and layout on strength, deformation, and final fracture behavior of titanium alloy gusset plates under the influence of an external load is presented and discussed. Several plates having differences in both the number and layout of the bolt holes were precision machined and then deformed under quasi-static loading. The specific influence of number of bolt holes and their layout on maximum load-carrying capability and even fracture load was determined. The conjoint influence of bolt number, bolt layout pattern, nature of loading, contribution from local stress concentration, and intrinsic microstructural effects in governing the macroscopic fracture mode and intrinsic microscopic mechanisms is presented and discussed.