We present three dinucleotide and six tetranucleotide microsatellite loci that were developed for the Brazilian free-tailed bat, Tadarida brasiliensis (Chiroptera, Molossidae). Ninety-one individuals from two populations were scored at each locus, revealing extremely high levels of polymorphism (15 –55 alleles per locus). These loci provide genetic markers for studying gene flow, migration and mating behaviour.